ANCOM Volcano Plot


ANCOM statistical results

W
Veillonella multispecies_spp8_2 (SPP8) 180
Escherichia coli (SP116) 180
Schaalia odontolytica (SP67) 180
Streptococcus mitis (SP5) 180
Streptococcus gordonii (SP49) 180
Fusobacterium nucleatum (SP4) 180
Bacillus halotolerans (SP3) 180
Rothia dentocariosa (SP81) 179
Veillonella parvula (SP6) 179
Salmonella enterica (SP37) 179
Neisseria subflava (SP40) 178
Truepera radiovictrix (SP35) 178
Alloprevotella sp. HMT473 (SP119) 178
Porphyromonas gingivalis (SP1) 178
Saccharibacteria_(TM7)_[G-1] bacterium HMT957 (SP110) 178
Prevotella oris (SP73) 177
Prevotella melaninogenica (SP23) 177
Streptococcus oralis (SP32) 177
Capnocytophaga sputigena (SP74) 176
Schaalia sp. HMT180 (SP84) 176
Streptococcus sanguinis (SP12) 176
Listeria monocytogenes (SP28) 175
Enterococcus faecalis (SP123) 175
Streptococcus sp. HMT064 (SP45) 174
Staphylococcus warneri (SP52) 174
Escherichia fergusonii (SP64) 174
Leptotrichia sp. HMT392 (SP113) 174
Pseudomonas aeruginosa (SP124) 174
Limosilactobacillus fermentum (SP8) 174
Shigella sonnei (SP83) 174
Lautropia mirabilis (SP39) 173
Aeromicrobium panaciterrae_nov_96.327% (SPN27) 173
Nocardiopsis nikkonensis_nov_97.250% (SPN50) 173
Erythrobacter aureus (SP151) 173
Abiotrophia defectiva (SP63) 172
Frondibacter mangrovi_nov_92.771% (SPN11) 172
Staphylococcus multispecies_spp2_3 (SPP2) 172
Peptoanaerobacter [Eubacterium] yurii (SP77) 170
Leptotrichia sp. HMT212 (SP54) 170
Ottowia sp. HMT894 (SP69) 169
Prevotella nigrescens (SP50) 169
Neisseria sicca (SP147) 168
Actinomyces sp. HMT448 (SP105) 168
Ruminococcaceae_[G-1] bacterium HMT075 (SP59) 167
Campylobacter showae (SP29) 167
Selenomonas artemidis (SP134) 167
Actinomyces sp. HMT169 (SP99) 166
Streptococcus intermedius (SP31) 166
Capnocytophaga gingivalis (SP103) 166
Leptotrichia wadei (SP72) 166
Campylobacter gracilis (SP82) 165
Actinomyces sp. HMT170 (SP22) 165
Capnocytophaga leadbetteri (SP16) 164
Granulicatella adiacens (SP19) 164
Bergeyella sp. HMT322 (SP133) 162
Kingella oralis (SP17) 162
Leptotrichia sp. HMT225 (SP143) 160
Actinomyces johnsonii (SP108) 160
Streptococcus sobrinus (SP111) 160
Gemella morbillorum (SP100) 160
Allobacillus halotolerans (SP9) 158
Prevotella sp. HMT475 (SP129) 158
Actinomyces massiliensis (SP94) 157
Cardiobacterium valvarum (SP78) 156
Actinomyces oris (SP146) 156
Porphyromonas pasteri (SP125) 155
Leptotrichia buccalis (SP47) 154
Scardovia wiggsiae (SP86) 154
Porphyromonas catoniae (SP131) 153
Lachnoanaerobaculum saburreum (SP106) 152
Alloprevotella tannerae (SP88) 152
Cutibacterium acnes (SP112) 149
Streptococcus salivarius (SP91) 148
Neisseria cinerea (SP178) 147
Schaalia meyeri (SP175) 146
Saccharibacteria_(TM7)_[G-6] bacterium HMT870 nov_97.093% (SPN15) 144
Peptidiphaga sp. HMT183 (SP107) 144
Campylobacter concisus (SP104) 143
Leptotrichia sp. HMT215 (SP55) 143
Haemophilus haemolyticus (SP161) 141
Lachnospiraceae_[G-3] bacterium HMT100 (SP121) 140
Saccharibacteria_(TM7)_[G-1] bacterium HMT348 (SP53) 137
Parvimonas micra (SP191) 135
Actinomyces sp. HMT175 (SP130) 135
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Percentile abundances of features by group

Percentile 0.0 25.0 50.0 75.0 100.0 0.0 25.0 50.0 75.0 100.0 0.0 25.0 50.0 75.0 100.0 0.0 25.0 50.0 75.0 100.0
Group OM OM OM OM OM SUPA SUPA SUPA SUPA SUPA SUPB SUPB SUPB SUPB SUPB ZM ZM ZM ZM ZM
Veillonella multispecies_spp8_2 (SPP8) 1.0 1.0 1.0 1.0 1.0 474.0 576.0 739.0 838.0 957.0 601.0 833.0 975.0 1218.0 1598.0 1.0 1.0 1.0 1.0 1.0
Escherichia coli (SP116) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 26.0 82.0 93.0 110.0
Schaalia odontolytica (SP67) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 11.0 265.0 299.0 520.0 630.0 935.0 1.0 1.0 1.0 1.0 1.0
Streptococcus mitis (SP5) 1.0 1.0 1.0 1.0 15.0 416.0 564.0 680.0 753.0 807.0 215.0 248.0 286.0 295.0 356.0 1.0 1.0 1.0 1.0 1.0
Streptococcus gordonii (SP49) 1.0 1.0 1.0 1.0 1.0 143.0 149.0 155.0 166.0 258.0 84.0 151.0 181.0 189.0 258.0 1.0 1.0 1.0 1.0 1.0
Fusobacterium nucleatum (SP4) 1.0 1.0 95.0 123.0 137.0 263.0 299.0 406.0 421.0 462.0 116.0 151.0 163.0 170.0 185.0 1.0 1.0 1.0 1.0 1.0
Bacillus halotolerans (SP3) 1.0 1.0 1.0 1.0 157.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1265.0 1586.0 2060.0 3291.0 3424.0
Rothia dentocariosa (SP81) 1.0 1.0 1.0 1.0 1.0 133.0 152.0 236.0 240.0 271.0 40.0 60.0 73.0 77.0 97.0 1.0 1.0 1.0 1.0 1.0
Veillonella parvula (SP6) 1.0 1.0 1.0 1.0 15.0 70.0 129.0 158.0 179.0 274.0 534.0 746.0 847.0 1248.0 1411.0 1.0 1.0 1.0 1.0 1.0
Salmonella enterica (SP37) 1.0 1.0 1.0 1.0 135.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 681.0 974.0 1262.0 2081.0 2297.0
Neisseria subflava (SP40) 1.0 1.0 362.0 446.0 651.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Truepera radiovictrix (SP35) 1.0 26.0 1532.0 2418.0 4632.0 1.0 1.0 1.0 1.0 3.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Alloprevotella sp. HMT473 (SP119) 1.0 1.0 1.0 1.0 1.0 108.0 133.0 164.0 190.0 213.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Porphyromonas gingivalis (SP1) 20.0 39.0 7382.0 8176.0 9640.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Saccharibacteria_(TM7)_[G-1] bacterium HMT957 (SP110) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 143.0 153.0 160.0 171.0 189.0 1.0 1.0 1.0 1.0 1.0
Prevotella oris (SP73) 1.0 1.0 648.0 741.0 909.0 1.0 33.0 37.0 42.0 55.0 1.0 1.0 1.0 37.0 46.0 1.0 1.0 1.0 1.0 1.0
Prevotella melaninogenica (SP23) 1.0 1.0 1.0 1.0 1.0 60.0 83.0 88.0 113.0 135.0 72.0 96.0 103.0 141.0 170.0 1.0 1.0 1.0 1.0 1.0
Streptococcus oralis (SP32) 1.0 1.0 1.0 1.0 1.0 90.0 158.0 212.0 239.0 267.0 1.0 38.0 45.0 45.0 53.0 1.0 1.0 1.0 1.0 1.0
Capnocytophaga sputigena (SP74) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 75.0 77.0 106.0 141.0 162.0 1.0 1.0 1.0 1.0 1.0
Schaalia sp. HMT180 (SP84) 1.0 1.0 1.0 1.0 1.0 45.0 87.0 99.0 104.0 155.0 38.0 48.0 91.0 98.0 135.0 1.0 1.0 1.0 1.0 1.0
Streptococcus sanguinis (SP12) 1.0 1.0 1.0 1.0 1.0 1.0 65.0 92.0 96.0 105.0 200.0 220.0 380.0 398.0 577.0 1.0 1.0 1.0 1.0 1.0
Listeria monocytogenes (SP28) 1.0 1.0 1.0 1.0 14.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 200.0 226.0 672.0 781.0 787.0
Enterococcus faecalis (SP123) 1.0 1.0 1.0 1.0 23.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 149.0 211.0 429.0 495.0 679.0
Streptococcus sp. HMT064 (SP45) 1.0 1.0 1.0 1.0 1.0 87.0 103.0 137.0 143.0 165.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Staphylococcus warneri (SP52) 1.0 1.0 42.0 80.0 120.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Escherichia fergusonii (SP64) 1.0 1.0 1.0 1.0 67.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 147.0 227.0 353.0 380.0 454.0
Leptotrichia sp. HMT392 (SP113) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 26.0 55.0 82.0 90.0 125.0 1.0 1.0 1.0 1.0 1.0
Pseudomonas aeruginosa (SP124) 1.0 1.0 1.0 1.0 24.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 210.0 231.0 316.0 500.0 507.0
Limosilactobacillus fermentum (SP8) 1.0 1.0 1.0 1.0 26.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 142.0 230.0 451.0 718.0 860.0
Shigella sonnei (SP83) 1.0 1.0 1.0 1.0 59.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 118.0 270.0 340.0 469.0 565.0
Lautropia mirabilis (SP39) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 9.0 13.0 14.0 115.0 117.0 150.0 158.0 206.0 1.0 1.0 1.0 1.0 1.0
Aeromicrobium panaciterrae_nov_96.327% (SPN27) 1.0 1.0 39.0 49.0 74.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Nocardiopsis nikkonensis_nov_97.250% (SPN50) 1.0 1.0 42.0 46.0 57.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Erythrobacter aureus (SP151) 1.0 3.0 39.0 53.0 70.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Abiotrophia defectiva (SP63) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 12.0 41.0 106.0 132.0 158.0 234.0 1.0 1.0 1.0 1.0 1.0
Frondibacter mangrovi_nov_92.771% (SPN11) 727.0 1033.0 1114.0 3713.0 8790.0 1.0 1.0 1.0 181.0 367.0 1.0 1.0 1.0 152.0 293.0 1.0 1.0 1.0 90.0 236.0
Staphylococcus multispecies_spp2_3 (SPP2) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 81.0 128.0 1013.0 1125.0 1612.0
Peptoanaerobacter [Eubacterium] yurii (SP77) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 4.0 18.0 21.0 26.0 30.0 37.0 1.0 1.0 1.0 1.0 1.0
Leptotrichia sp. HMT212 (SP54) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 25.0 81.0 117.0 133.0 161.0 179.0 1.0 1.0 1.0 1.0 1.0
Ottowia sp. HMT894 (SP69) 1.0 1.0 1.0 1.0 1.0 1.0 12.0 16.0 23.0 29.0 30.0 47.0 59.0 62.0 81.0 1.0 1.0 1.0 1.0 1.0
Prevotella nigrescens (SP50) 1.0 1.0 1.0 1.0 1.0 1.0 38.0 53.0 61.0 75.0 48.0 52.0 56.0 69.0 91.0 1.0 1.0 1.0 1.0 1.0
Neisseria sicca (SP147) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 15.0 20.0 28.0 84.0 101.0 108.0 129.0 159.0 1.0 1.0 1.0 1.0 1.0
Actinomyces sp. HMT448 (SP105) 1.0 1.0 1.0 1.0 1.0 40.0 66.0 100.0 145.0 196.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Ruminococcaceae_[G-1] bacterium HMT075 (SP59) 1.0 1.0 1.0 1.0 1.0 1.0 10.0 11.0 16.0 22.0 35.0 39.0 49.0 53.0 63.0 1.0 1.0 1.0 1.0 1.0
Campylobacter showae (SP29) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 19.0 29.0 33.0 40.0 43.0 49.0 1.0 1.0 1.0 1.0 1.0
Selenomonas artemidis (SP134) 1.0 1.0 1.0 1.0 1.0 17.0 31.0 45.0 52.0 57.0 1.0 13.0 21.0 28.0 30.0 1.0 1.0 1.0 1.0 1.0
Actinomyces sp. HMT169 (SP99) 1.0 1.0 1.0 1.0 1.0 1.0 58.0 75.0 100.0 112.0 56.0 70.0 87.0 110.0 139.0 1.0 1.0 1.0 1.0 1.0
Streptococcus intermedius (SP31) 1.0 1.0 1.0 1.0 1.0 1.0 12.0 30.0 38.0 43.0 11.0 32.0 36.0 46.0 62.0 1.0 1.0 1.0 1.0 1.0
Capnocytophaga gingivalis (SP103) 1.0 1.0 1.0 1.0 1.0 1.0 35.0 38.0 44.0 102.0 43.0 60.0 65.0 84.0 134.0 1.0 1.0 1.0 1.0 1.0
Leptotrichia wadei (SP72) 1.0 1.0 1.0 1.0 1.0 87.0 122.0 129.0 167.0 217.0 1.0 1.0 36.0 41.0 52.0 1.0 1.0 1.0 1.0 1.0
Campylobacter gracilis (SP82) 1.0 1.0 1.0 1.0 1.0 46.0 74.0 75.0 85.0 112.0 1.0 9.0 18.0 28.0 34.0 1.0 1.0 1.0 1.0 1.0
Actinomyces sp. HMT170 (SP22) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 29.0 30.0 36.0 81.0 1.0 1.0 1.0 1.0 1.0
Capnocytophaga leadbetteri (SP16) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 41.0 52.0 67.0 133.0 177.0 188.0 223.0 237.0 1.0 1.0 1.0 1.0 1.0
Granulicatella adiacens (SP19) 1.0 1.0 1.0 1.0 1.0 43.0 112.0 168.0 199.0 208.0 1.0 103.0 124.0 130.0 144.0 1.0 1.0 1.0 1.0 1.0
Bergeyella sp. HMT322 (SP133) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 17.0 21.0 25.0 14.0 22.0 28.0 35.0 49.0 1.0 1.0 1.0 1.0 1.0
Kingella oralis (SP17) 1.0 1.0 1.0 1.0 1.0 32.0 46.0 49.0 60.0 68.0 1.0 3.0 7.0 13.0 26.0 1.0 1.0 1.0 1.0 1.0
Leptotrichia sp. HMT225 (SP143) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 14.0 21.0 28.0 33.0 1.0 1.0 1.0 1.0 1.0
Actinomyces johnsonii (SP108) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 6.0 1.0 6.0 17.0 27.0 41.0 1.0 1.0 1.0 1.0 1.0
Streptococcus sobrinus (SP111) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 15.0 57.0 138.0 165.0 1.0 1.0 1.0 1.0 1.0
Gemella morbillorum (SP100) 1.0 1.0 1.0 1.0 1.0 1.0 12.0 29.0 32.0 41.0 1.0 38.0 67.0 74.0 88.0 1.0 1.0 1.0 1.0 1.0
Allobacillus halotolerans (SP9) 36.0 95.0 160.0 647.0 4180.0 1.0 1.0 1.0 84.0 224.0 1.0 1.0 1.0 44.0 150.0 1.0 1.0 1.0 13.0 145.0
Prevotella sp. HMT475 (SP129) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 9.0 20.0 23.0 36.0 1.0 1.0 1.0 1.0 1.0
Actinomyces massiliensis (SP94) 1.0 1.0 1.0 1.0 1.0 33.0 47.0 79.0 82.0 96.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Cardiobacterium valvarum (SP78) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 10.0 11.0 12.0 14.0 1.0 1.0 1.0 1.0 1.0
Actinomyces oris (SP146) 1.0 1.0 1.0 1.0 1.0 1.0 16.0 21.0 32.0 43.0 1.0 53.0 64.0 80.0 166.0 1.0 1.0 1.0 1.0 1.0
Porphyromonas pasteri (SP125) 1.0 1.0 1.0 1.0 1.0 39.0 48.0 62.0 80.0 91.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Leptotrichia buccalis (SP47) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 35.0 53.0 63.0 20.0 26.0 39.0 52.0 59.0 1.0 1.0 1.0 1.0 1.0
Scardovia wiggsiae (SP86) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 10.0 12.0 23.0 1.0 1.0 1.0 1.0 1.0
Porphyromonas catoniae (SP131) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 19.0 1.0 15.0 17.0 20.0 24.0 1.0 1.0 1.0 1.0 1.0
Lachnoanaerobaculum saburreum (SP106) 1.0 1.0 1.0 1.0 1.0 37.0 57.0 58.0 60.0 68.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Alloprevotella tannerae (SP88) 1.0 1.0 1.0 1.0 1.0 35.0 41.0 52.0 68.0 81.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Cutibacterium acnes (SP112) 1.0 1.0 1.0 29.0 81.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Streptococcus salivarius (SP91) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 22.0 34.0 1.0 46.0 84.0 135.0 141.0 1.0 1.0 1.0 1.0 1.0
Neisseria cinerea (SP178) 1.0 1.0 1.0 1.0 1.0 29.0 47.0 56.0 62.0 69.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Schaalia meyeri (SP175) 1.0 1.0 1.0 1.0 12.0 40.0 63.0 70.0 83.0 101.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Saccharibacteria_(TM7)_[G-6] bacterium HMT870 nov_97.093% (SPN15) 1.0 1.0 1.0 1.0 1.0 31.0 40.0 53.0 60.0 73.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Peptidiphaga sp. HMT183 (SP107) 1.0 1.0 1.0 1.0 1.0 25.0 28.0 63.0 70.0 106.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Campylobacter concisus (SP104) 1.0 1.0 1.0 1.0 1.0 33.0 38.0 49.0 53.0 78.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Leptotrichia sp. HMT215 (SP55) 1.0 1.0 1.0 1.0 1.0 27.0 36.0 39.0 52.0 54.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Haemophilus haemolyticus (SP161) 1.0 1.0 1.0 1.0 1.0 31.0 32.0 37.0 42.0 60.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Lachnospiraceae_[G-3] bacterium HMT100 (SP121) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 13.0 20.0 1.0 11.0 12.0 19.0 22.0 1.0 1.0 1.0 1.0 1.0
Saccharibacteria_(TM7)_[G-1] bacterium HMT348 (SP53) 1.0 1.0 1.0 1.0 1.0 22.0 25.0 36.0 48.0 51.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Parvimonas micra (SP191) 1.0 1.0 1.0 1.0 1.0 18.0 23.0 25.0 30.0 34.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
Actinomyces sp. HMT175 (SP130) 1.0 1.0 1.0 1.0 1.0 65.0 71.0 76.0 103.0 117.0 1.0 1.0 1.0 1.0 38.0 1.0 1.0 1.0 1.0 1.0
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