Table of Content
I.	Project Summary
II.	Workflow Checklist
III.	NGS Sequencing Results
IV.	Complete Report Download
V.	Raw Sequence Data Download
VI.	Analysis - DADA2 Read Processing
	Sample Meta Info
	Read Count by Sample
VII.	Analysis - Read Taxonomy Assignment
	Taxonomy Barplots
VIII.	Analysis - Alpha Diversity
IX.	Analysis - Beta Diversity
X.	Analysis - Differential Abundance
	ANCOM Result
	LEfSe Result
XI.	Analysis - Heatmap Profile
XII.	Analysis - Network Association

(Click to navigate)

FOMC Service Report

16S rRNA Gene V1V3 Amplicon Sequencing

Version V1.41 fork

Version History

The Forsyth Institute, Cambridge, MA, USA

August 25, 2025

Project ID: 20250807_obesity

I. Project Summary

Project 20250807_obesity services do not include NGS sequencing of the V1V3 region of the 16S rRNA gene amplicons from the samples. First and foremost, please download this report. These links will expire after 60 days. We cannot guarantee the availability of your data after 60 days.

Full Bioinformatics analysis service was requested.

We provide many downstream analyses such as taxonomy assignment, alpha and beta diversity analyses, and differential abundance analysis.

For taxonomy assignment, most informative would be the taxonomy barplots. We provide an interactive barplots to show the relative abundance of microbes at different taxonomy levels (from Phylum to species) that you can choose.

If you specify which groups of samples you want to compare for differential abundance, we provide both ANCOM and LEfSe differential abundance analysis.

II. Workflow Checklist

☑	1.	Sample Received
☑	2.	Sample Quality Evaluated
☑	3.	Sample Prepared for Sequencing
☑	4.	Next-Gen Sequencing
☑	5.	Sequence Quality Check
☑	6.	Absolute Abundance
☑	7.	Report and Raw Sequence Data Available for Download
☑	8.	Bioinformatics Analysis - Reads Processing (DADA2 Quality Trimming, Denoising, Paired Reads Merging)
☑	9.	Bioinformatics Analysis - Reads Taxonomy Assignment
☑	10.	Bioinformatics Analysis - Alpha Diversity Analysis
☑	11.	Bioinformatics Analysis - Beta Diversity Analysis
☑	12.	Bioinformatics Analysis - Differential Abundance Analysis
☑	13.	Bioinformatics Analysis - Heatmap Profile
☑	14.	Bioinformatics Analysis - Network Association

III. NGS Sequencing

Not available

IV. Complete Report Download

The complete report of your project, including all links in this report, can be downloaded by clicking the link provided below. The downloaded file is a compressed ZIP file and once unzipped, open the file “REPORT.html” (may only shown as "REPORT" in your computer) by double clicking it. Your default web browser will open it and you will see the exact content of this report.

Please download and save the file to your computer storage device. The download link will expire after 60 days upon your receiving of this report.

Complete report download link:

To view the report, please follow the following steps:

1. Download the .zip file from the report link above.

2. Extract all the contents of the downloaded .zip file to your desktop.

3. Open the extracted folder and find the "REPORT.html" (may shown as only "REPORT").

4. Open (double-clicking) the REPORT.html file. Your default browser will open the top age of the complete report. Within the report, there are links to view all the analyses performed for the project.

V. Raw Sequence Data Download

Not available

VI. Analysis - DADA2 Read Processing

Not available

Sample Meta Information

#SampleID	SampleName	Group	Age	Gender	BMI	Smoking	Symptoms	MouthUlcer	PainfulGums	BleedingGums	LooseTeeth	Toothache	Denture	ALT	AST	HDL	LDL_Calc	Triglyceride	Cholesterol	HbA1C	DiastolicBP	SystolicBP	PulseRate
O1	O1	case	29	Male	38.02	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	NA	NA	NA
O2	O2	case	25	Male	30.3	NA	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	NA	NA	NA
O3	O3	case	30	Female	39.98	no	yes	no	yes	yes	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	High	High	Normal
O4	O4	case	29	Female	35.22	no	yes	no	no	yes	no	yes	no	Normal	Normal	Low	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O5	O5	case	32	Male	32.74	no	no	no	no	no	no	no	no	High	High	Low	High	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O6	O6	case	30	Female	32.76	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
O7	O7	case	26	Male	43.43	no	no	no	no	no	no	no	no	High	High	Low	High	Normal	BorderlineHigh	Normal	NA	NA	NA
O11	O11	case	29	Male	33.35	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O13	O13	case	23	Male	32.55	yes	no	no	no	no	no	no	yes	Normal	Normal	Desirable	High	BorderlineHigh	BorderlineHigh	Normal	Normal	Normal	Normal
O36	O36	case	30	Female	31.73	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O38	O38	case	28	Female	35.46	no	yes	no	no	yes	no	no	no	Normal	Normal	Low	Very high	BorderlineHigh	BorderlineHigh	Normal	Normal	Normal	Normal
O40	O40	case	30	Male	34.6	yes	no	no	no	no	no	no	no	Normal	Normal	Desirable	Very high	High	High	Normal	Normal	Normal	Normal
O45	O45	case	28	Male	38.41	no	no	no	no	no	no	no	no	High	Normal	Low	High	Normal	BorderlineHigh	Normal	High	High	Normal
O46	O46	case	20	Male	33.76	no	no	no	no	no	no	no	no	Normal	Normal	Low	Optimal	Normal	Normal	Normal	Normal	Normal	Normal
O47	O47	case	24	Female	32.35	no	yes	no	no	no	no	yes	yes	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O48	O48	case	31	Female	33.06	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	Normal	High	Normal
O49	O49	case	20	Female	31.09	no	yes	no	no	no	no	yes	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O50	O50	case	20	Male	30.91	no	no	no	no	no	no	no	no	Normal	Normal	Low	High	Normal	Normal	Normal	Normal	Normal	Normal
O51	O51	case	32	Female	35.83	no	no	no	no	no	no	yes	no	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O52	O52	case	22	Female	32.9	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	High	Normal
O54	O54	case	21	Female	30.69	no	no	no	no	no	no	no	no	Normal	Normal	Low	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O55	O55	case	27	Male	31.68	yes	no	no	no	no	no	no	yes	Normal	Normal	Desirable	Very high	High	High	Normal	Normal	Normal	Normal
O56	O56	case	31	Female	35.39	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O57	O57	case	29	Male	30.16	no	no	no	no	no	no	no	no	High	High	Low	High	Normal	Normal	Normal	Normal	Normal	Normal
O58	O58	case	21	Male	36.07	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	High	Normal
O59	O59	case	28	Female	42.65	no	yes	no	no	no	no	yes	no	Normal	Normal	Desirable	Optimal	Normal	Normal	Normal	Normal	Normal	Normal
O61	O61	case	33	Male	31.18	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O63	O63	case	23	Female	34.85	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O65	O65	case	32	Male	42.86	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O67	O67	case	26	Male	30.56	no	yes	no	no	no	no	yes	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O69	O69	case	25	Female	35.64	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
O71	O71	case	26	Male	38.27	yes	no	no	no	no	no	no	no	Normal	Normal	Low	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
O73	O73	case	30	Female	46.49	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
O75	O75	case	27	Male	31.65	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O78	O78	case	30	Female	31.4	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	BorderlineHigh	Normal	Normal	Normal	Normal	Normal
O80	O80	case	34	Female	31.46	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	High
O82	O82	case	25	Male	32.55	no	no	no	no	no	no	no	no	High	Normal	Desirable	High	Normal	BorderlineHigh	Normal	High	High	Normal
O84	O84	case	30	male	47.82	no	no	no	no	no	no	no	no	Normal	Normal	Low	High	BorderlineHigh	BorderlineHigh	Normal	High	High	High
O86	O86	case	25	Male	39.08	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
O88	O88	case	34	Female	39.68	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
O90	O90	case	33	Male	31.57	yes	no	no	no	no	no	no	no	High	Normal	Low	High	Normal	Normal	Normal	Normal	High	Normal
O92	O92	control	24	Male	32.46	yes	no	no	no	no	no	no	no	High	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C14	C14	control	25	Male	21.4	yes	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C15	C15	control	31	Male	19.74	no	yes	no	no	yes	no	yes	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	NA	NA	NA
C16	C16	control	25	Female	19	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C18	C18	control	31	Female	24.65	no	no	no	yes	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C20	C20	control	26	Male	20.21	yes	yes	no	no	no	no	yes	no	Normal	Normal	Desirable	High	Normal	Normal	Normal	NA	NA	NA
C22	C22	control	27	Female	24.27	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C24	C24	control	22	Female	22.13	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	BorderlineHigh	BorderlineHigh	Normal	Normal	Normal	Normal
C26	C26	control	28	Male	23.63	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	High	BorderlineHigh	Normal	Normal	Normal	High
C27	C27	control	25	Male	21.53	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	NA	NA	NA
C28	C28	control	26	Male	19.94	yes	no	no	no	no	no	no	no	Normal	Normal	Desirable	High	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C29	C29	control	21	Male	23.66	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C31	C31	control	23	Male	23.16	yes	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C32	C32	control	30	Female	24.74	no	no	no	no	no	no	no	no	High	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C33	C33	control	33	Female	22.64	no	no	no	no	no	no	no	no	Normal	Normal	Low	NearOptimal	BorderlineHigh	Normal	Normal	Normal	Normal	Normal
C34	C34	control	28	Female	23.38	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C35	C35	control	30	Male	24.61	no	yes	no	no	no	no	yes	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C37	C37	control	20	Male	21.11	yes	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C39	C39	control	24	Female	20.7	no	yes	no	no	no	no	yes	yes	Normal	Normal	Low	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C41	C41	control	33	Female	23.96	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C42	C42	control	21	Female	21	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C43	C43	control	33	Female	23.61	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C44	C44	control	30	Male	24.7	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	Optimal	Normal	Normal	Normal	Normal	Normal	Normal
C60	C60	control	26	Female	18.68	no	no	no	no	no	no	no	yes	Normal	High	Desirable	Optimal	Normal	Normal	Normal	Normal	Normal	Normal
C62	C62	control	27	Male	22.38	no	no	no	no	yes	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C64	C64	control	22	Female	19.95	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C66	C66	control	31	Male	23.52	yes	no	no	no	no	no	no	yes	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C68	C68	control	27	Male	24.19	no	no	no	no	no	no	no	yes	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C70	C70	control	21	Female	21.03	no	no	no	no	no	no	no	no	Normal	Normal	Low	High	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C72	C72	control	26	Male	23.47	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	Normal	Normal	Normal	Normal	Normal
C74	C74	control	32	Female	20.73	no	no	no	no	yes	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C76	C76	control	31	Male	19.5	no	yes	no	no	no	no	yes	yes	Normal	Normal	Desirable	BorderlineHigh	BorderlineHigh	BorderlineHigh	Normal	Normal	Normal	Normal
C77	C77	control	26	Male	19.67	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C79	C79	control	30	Female	23.71	no	yes	no	yes	yes	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C81	C81	control	33	Female	23.12	no	no	no	no	no	no	no	no	Normal	Normal	Low	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C83	C83	control	23	Female	24.31	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	NearOptimal	Normal	BorderlineHigh	Normal	Normal	High	Normal
C85	C85	control	32	Male	18.52	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C87	C87	control	25	Male	18.52	yes	no	no	no	no	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
C89	C89	control	34	Female	23.81	no	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	Normal	Normal
C91	C91	control	31	male	23.25	yes	no	no	no	no	no	no	yes	Normal	Normal	Desirable	BorderlineHigh	Normal	BorderlineHigh	Normal	Normal	High	Normal
C93	C93	control	23	Male	24.73	no	no	no	no	no	no	no	no	High	High	Desirable	Optimal	Normal	Normal	Normal	Normal	Normal	Normal
C94	C94	control	25	Male	20.26	yes	no	no	no	no	no	no	no	Normal	Normal	Desirable	BorderlineHigh	Normal	Normal	Normal	Normal	Normal	Normal
NC1	NC1	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
NC2	NC2	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
NC3	NC3	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA

ASV Read Counts by Samples

#Sample ID	Read Count
NC2	3,966
NC1	5,866
C89	15,273
NC3	18,422
O57	22,696
O54	23,472
O92	23,556
O84	24,433
O65	25,781
O90	26,572
C77	28,010
O51	29,709
C64	30,114
C43	30,141
O45	30,737
O55	31,116
O67	31,748
O11	32,164
C31	32,560
O86	33,445
O71	34,024
O6	34,127
C37	34,979
C68	36,331
C76	37,027
C41	37,120
C44	37,859
O48	38,175
C26	38,569
O69	38,796
O4	38,890
O1	39,020
C34	39,034
C83	39,117
C20	39,131
C18	39,654
O38	39,682
O50	39,982
O36	40,017
C72	40,156
O61	40,243
O49	40,375
O58	40,752
O46	41,044
C35	41,805
C66	41,805
O3	41,818
O5	42,574
O40	42,586
C87	42,975
O59	42,994
C60	43,163
C32	43,807
C14	43,998
O63	44,124
O52	44,890
O47	45,283
C28	45,349
C22	45,410
O88	46,127
C85	46,137
C79	46,583
C39	46,947
C94	47,333
C81	47,697
O13	48,405
C15	49,384
O7	49,937
C33	50,180
C74	50,685
C42	50,740
C70	50,795
C62	50,967
C27	52,896
C29	53,035
O2	54,234
O75	54,685
O73	55,809
O56	57,228
C93	58,783
O78	59,558
C91	60,335
C24	61,567
C16	63,206
O80	69,332
O82	69,925

VII. Analysis - Read Taxonomy Assignment

Read Taxonomy Assignment - Methods

The species-level, open-reference 16S rRNA NGS reads taxonomy assignment pipeline

Version 20210310

1. Raw sequences reads in FASTA format were BLASTN-searched against a combined set of 16S rRNA reference sequences. It consists of MOMD (version 0.1), the HOMD (version 15.2 http://www.homd.org/index.php?name=seqDownload&file&type=R ), HOMD 16S rRNA RefSeq Extended Version 1.1 (EXT), GreenGene Gold (GG) (http://greengenes.lbl.gov/Download/Sequence_Data/Fasta_data_files/gold_strains_gg16S_aligned.fasta.gz) , and the NCBI 16S rRNA reference sequence set (https://ftp.ncbi.nlm.nih.gov/blast/db/16S_ribosomal_RNA.tar.gz). These sequences were screened and combined to remove short sequences (<1000nt), chimera, duplicated and sub-sequences, as well as sequences with poor taxonomy annotation (e.g., without species information). This process resulted in 1,015 from HOMD V15.22, 495 from EXT, 3,940 from GG and 18,044 from NCBI, a total of 25,120 sequences. Altogether these sequence represent a total of 15,601 oral and non-oral microbial species.

The NCBI BLASTN version 2.7.1+ (Zhang et al, 2000) was used with the default parameters. Reads with ≥ 98% sequence identity to the matched reference and ≥ 90% alignment length (i.e., ≥ 90% of the read length that was aligned to the reference and was used to calculate the sequence percent identity) were classified based on the taxonomy of the reference sequence with highest sequence identity. If a read matched with reference sequences representing more than one species with equal percent identity and alignment length, it was subject to chimera checking with USEARCH program version v8.1.1861 (Edgar 2010). Non-chimeric reads with multi-species best hits were considered valid and were assigned with a unique species notation (e.g., spp) denoting unresolvable multiple species.

2. Unassigned reads (i.e., reads with < 98% identity or < 90% alignment length) were pooled together and reads < 200 bases were removed. The remaining reads were subject to the de novo operational taxonomy unit (OTU) calling and chimera checking using the USEARCH program version v8.1.1861 (Edgar 2010). The de novo OTU calling and chimera checking was done using 98% as the sequence identity cutoff, i.e., the species-level OTU. The output of this step produced species-level de novo clustered OTUs with 98% identity. Representative reads from each of the OTUs/species were then BLASTN-searched against the same reference sequence set again to determine the closest species for these potential novel species. These potential novel species were pooled together with the reads that were signed to specie-level in the previous step, for down-stream analyses.

Reference:
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010 Oct 1;26(19):2460-1. doi: 10.1093/bioinformatics/btq461. Epub 2010 Aug 12. PubMed PMID: 20709691.

3. Designations used in the taxonomy:

	1) Taxonomy levels are indicated by these prefixes:
	
	   k__: domain/kingdom
	   p__: phylum
	   c__: class
	   o__: order
	   f__: family
	   g__: genus  
	   s__: species
	
	   Example: 
	
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Blautia;s__faecis
		
	2) Unique level identified – known species:
	   
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__hominis
	
	   The above example shows some reads match to a single species (all levels are unique)
	
	3) Non-unique level identified – known species:

	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__multispecies_spp123_3
	   
	   The above example “s__multispecies_spp123_3” indicates certain reads equally match to 3 species of the 
	   genus Roseburia; the “spp123” is a temporally assigned species ID.
	
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__multigenus;s__multispecies_spp234_5
	   
	   The above example indicates certain reads match equally to 5 different species, which belong to multiple genera.; 
	   the “spp234” is a temporally assigned species ID.
	
	4) Unique level identified – unknown species, potential novel species:
	   
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__ hominis_nov_97%
	   
	   The above example indicates that some reads have no match to any of the reference sequences with 
	   sequence identity ≥ 98% and percent coverage (alignment length)  ≥ 98% as well. However this groups 
	   of reads (actually the representative read from a de novo  OTU) has 96% percent identity to 
	   Roseburia hominis, thus this is a potential novel species, closest to Roseburia hominis. 
	   (But they are not the same species).
	
	5) Multiple level identified – unknown species, potential novel species:
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__ multispecies_sppn123_3_nov_96%
	
	   The above example indicates that some reads have no match to any of the reference sequences 
	   with sequence identity ≥ 98% and percent coverage (alignment length)  ≥ 98% as well. 
	   However this groups of reads (actually the representative read from a de novo  OTU) 
	   has 96% percent identity equally to 3 species in Roseburia. Thus this is no single 
	   closest species, instead this group of reads match equally to multiple species at 96%. 
	   Since they have passed chimera check so they represent a novel species. “sppn123” is a 
	   temporary ID for this potential novel species.

4. The taxonomy assignment algorithm is illustrated in this flow char below:

Read Taxonomy Assignment - Result Summary *

Code	Category	MPC=0% (>=1 read)	MPC=0.01%(>=351 reads)
A	Total reads	3,528,976	3,528,976
B	Total assigned reads	3,516,308	3,516,308
C	Assigned reads in species with read count < MPC	0	29,010
D	Assigned reads in samples with read count < 500	0	0
E	Total samples	86	86
F	Samples with reads >= 500	86	86
G	Samples with reads < 500	0	0
H	Total assigned reads used for analysis (B-C-D)	3,516,308	3,487,298
I	Reads assigned to single species	3,352,786	3,332,901
J	Reads assigned to multiple species	103,739	102,770
K	Reads assigned to novel species	59,783	51,627
L	Total number of species	449	242
M	Number of single species	336	202
N	Number of multi-species	14	8
O	Number of novel species	99	32
P	Total unassigned reads	12,668	12,668
Q	Chimeric reads	569	569
R	Reads without BLASTN hits	0	0
S	Others: short, low quality, singletons, etc.	12,099	12,099
	A=B+P=C+D+H+Q+R+S
	E=F+G
	B=C+D+H
	H=I+J+K
	L=M+N+O
	P=Q+R+S

* MPC = Minimal percent (of all assigned reads) read count per species, species with read count < MPC were removed.

* Samples with reads < 500 were removed from downstream analyses.

* The assignment result from MPC=0.1% was used in the downstream analyses.

Read Taxonomy Assignment - ASV Species-Level Read Counts Table

This table shows the read counts for each sample (columns) and each species identified based on the ASV sequences. The downstream analyses were based on this table.

SPID	Taxonomy	C14	C15	C16	C18	C20	C22	C24	C26	C27	C28	C29	C31	C32	C33	C34	C35	C37	C39	C41	C42	C43	C44	C60	C62	C64	C66	C68	C70	C72	C74	C76	C77	C79	C81	C83	C85	C87	C89	C91	C93	C94	NC1	NC2	NC3	O1	O11	O13	O2	O3	O36	O38	O4	O40	O45	O46	O47	O48	O49	O5	O50	O51	O52	O54	O55	O56	O57	O58	O59	O6	O61	O63	O65	O67	O69	O7	O71	O73	O75	O78	O80	O82	O84	O86	O88	O90	O92
SP1	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Oribacterium;asaccharolyticum	111	43	432	71	0	63	226	66	252	6	146	0	126	4	44	0	17	151	28	342	9	35	71	131	88	9	52	41	119	126	9	25	25	57	176	35	25	16	87	368	495	0	17	142	75	30	317	0	74	17	45	39	38	61	31	16	264	5	78	95	38	101	20	60	22	54	58	56	59	24	122	27	54	15	124	68	223	56	49	83	5	20	226	59	18	8
SP10	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;infantis	3214	251	2274	1055	31	5822	1707	135	334	473	1729	66	808	793	323	744	265	2414	461	823	158	628	1051	470	1385	1469	993	122	860	2439	314	74	957	715	517	886	2530	255	2373	2535	722	111	41	680	416	95	416	1811	911	1388	853	1887	709	1235	189	1200	497	702	365	2591	74	117	898	115	1278	1133	1313	1016	264	61	704	1019	147	980	414	1677	1810	530	449	1033	793	174	1274	452	647	52
SP101	Bacteria;Saccharibacteria_(TM7);TM7_[C-1];TM7_[O-1];TM7_[F-1];TM7_[G-3];sp._oral_taxon_351	117	505	0	8	1091	27	80	17	115	34	60	71	32	32	87	0	64	122	14	104	18	4	37	68	14	44	34	62	200	0	19	16	5	18	30	185	19	24	19	129	173	16	0	24	210	47	117	86	12	71	0	14	63	41	18	15	60	48	1176	34	11	212	20	19	13	5	53	26	5	13	130	17	27	7	829	9	19	6	4	15	7	58	33	45	16	2
SP102	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;meyeri	0	0	0	7	0	0	0	0	17	0	8	0	0	2	0	0	0	33	0	0	0	1	0	0	0	279	0	0	8	0	0	0	0	8	0	0	0	0	0	41	0	0	0	226	97	0	77	0	0	8	0	0	562	0	0	0	0	38	0	0	0	0	0	0	0	0	29	3	0	0	0	0	0	0	0	0	0	0	0	0	18	0	590	0	0	0
SP103	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._oral_taxon_275	0	225	0	33	0	8	0	0	0	0	0	0	46	0	0	0	0	2	0	0	0	0	0	0	0	0	0	23	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	10	0	0	0	0	0	0	0	80	1	11	2	0	0	37	350	2	51	0	0	0	0	0	17	0	7	30	0	2	4	0	0	0	0	370	0	0	0	2	0	37	0	0
SP104	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;sp._oral_taxon_478	27	32	9	2	0	2	20	6	11	14	5	28	2	1	6	2	0	1	28	0	41	8	0	0	0	0	1	2	20	5	0	0	1	61	177	0	0	6	2	0	4	6	0	6	40	16	0	5	9	0	2	4	19	11	0	9	24	0	246	46	92	144	0	31	1	0	0	17	18	79	99	2	36	0	64	4	47	161	0	198	2	6	7	50	0	4
SP105	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_431	723	463	917	188	0	1192	2888	231	584	320	4309	85	244	86	389	463	981	400	181	618	168	936	426	412	489	805	575	2268	401	2401	911	389	103	1661	245	2402	62	232	649	1266	407	38	48	117	302	408	140	376	426	555	415	472	522	390	79	192	161	856	306	29	447	1708	219	293	215	386	1001	190	427	0	1071	446	81	184	195	698	303	441	787	583	329	507	152	1078	336	224
SP106	Bacteria;Firmicutes;Bacilli;Lactobacillales;Carnobacteriaceae;Granulicatella;adiacens	2121	645	3966	1484	1390	2133	2291	520	1206	975	985	693	1078	519	254	2332	1138	1988	826	1305	328	2489	1136	808	1261	1846	489	7576	1021	1089	773	330	667	969	333	2295	1718	2051	1729	1077	644	94	123	581	291	647	476	1586	903	332	689	306	689	718	512	523	487	875	348	326	272	1506	2200	597	2138	905	1237	1263	575	564	1341	2403	881	1666	195	1187	1632	653	1961	672	936	111	375	1421	881	452
SP107	Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;haemolysans	22	187	159	658	172	198	559	386	27	91	131	117	160	553	688	444	80	45	138	254	317	227	135	50	16	154	68	53	143	143	122	19	131	4	28	273	186	21	194	49	77	39	21	97	9	125	46	714	98	52	20	16	20	99	151	201	80	381	18	15	51	235	98	227	319	83	246	72	57	123	213	96	325	265	45	117	105	110	97	17	717	77	15	107	324	142
SP109	Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;concisus	11	162	12	12	54	5	16	23	253	18	24	30	32	4	12	7	8	0	111	45	8	23	4	0	65	19	7	17	3	10	48	41	7	41	69	7	43	11	13	1	0	0	4	9	188	53	270	261	12	68	25	25	13	13	12	19	201	78	111	15	17	108	20	16	10	6	54	16	54	27	92	14	17	8	788	4	10	18	22	4	0	0	4	1	26	4
SP110	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;haemolyticus	0	26	0	3	58	0	4	5	2	0	0	0	0	0	7	0	0	0	34	0	0	0	0	0	0	0	4	0	2	0	4	0	0	0	0	0	0	0	0	3	0	0	0	0	3	1	0	48	29	0	0	51	0	0	19	41	4	591	0	0	0	0	0	17	0	0	236	0	0	3	0	0	24	0	11	0	2	15	0	0	32	0	0	2	2	0
SP111	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_417	1086	30	17	0	1	1	38	92	726	73	70	85	549	7	3077	3	161	324	1327	204	67	161	23	116	241	312	351	126	132	10	56	1860	5	687	530	39	98	78	126	289	1261	24	0	120	1372	643	866	14	70	35	57	136	17	34	57	8	294	129	389	546	1134	104	45	100	2	82	59	298	29	1026	73	47	136	2	149	120	45	3076	110	113	4	41	108	39	99	17
SP112	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;histicola	1649	8	7	235	67	5	292	1563	1258	4315	15	5298	48	10	1488	1220	793	106	836	1027	487	190	268	10	311	573	39	3	11	5	996	1062	745	1111	1069	7	711	125	396	56	2101	261	119	208	1368	1621	870	105	578	2292	2158	49	1481	327	21	176	98	98	227	17	674	464	1202	1580	187	176	144	131	97	783	335	1235	1209	131	48	238	1175	7294	482	1074	13	18	235	1254	5	1937
SP113	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;elongata	4	212	0	4	53	10	2	0	25	4	0	0	83	13	5	4	0	9	7	4	0	4	54	0	0	0	0	375	17	0	0	12	0	0	0	2	11	3	0	36	0	0	0	16	0	10	57	0	27	11	0	0	2	4	12	47	6	59	5	17	16	2	9	0	2	2	31	10	0	26	4	12	0	3	25	0	0	2	0	0	0	0	7	0	0	0
SP114	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Lachnoanaerobaculum;saburreum	55	0	0	0	4	0	17	27	0	0	45	0	9	6	39	0	1	14	1	0	4	5	1	163	0	5	0	1	9	0	0	6	0	6	34	0	0	0	0	43	0	0	0	4	0	0	0	0	2	0	0	3	0	2	0	2	95	5	19	0	11	10	0	0	10	0	2	0	1	6	2	0	0	8	0	0	0	6	16	17	0	6	2	0	0	0
SP115	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_212	0	106	13	19	0	6	8	0	34	12	2	0	107	12	25	4	2	66	7	6	16	0	28	41	6	12	2	0	27	27	3	9	6	1	60	70	23	7	0	18	0	0	0	8	0	12	7	49	21	0	4	9	2	43	0	13	4	23	6	2	34	0	4	7	0	2	16	2	0	40	0	5	6	0	24	4	9	72	8	6	2	24	0	10	3	0
SP116	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._oral_taxon_278	1	219	2	0	98	1	6	0	6	0	0	0	18	24	30	11	0	8	11	3	31	0	0	1	0	0	11	48	121	0	0	0	2	0	38	0	0	21	239	0	4	22	0	3	0	53	0	541	52	28	21	68	0	0	37	0	14	216	178	8	220	0	2	0	0	0	110	0	0	105	7	2	30	0	0	2	0	278	2	0	26	9	1	100	0	0
SP117	Bacteria;Proteobacteria;Betaproteobacteria;Burkholderiales;Comamonadaceae;Ottowia;sp._oral_taxon_894	0	8	0	0	0	205	0	0	12	0	0	0	0	1	2	0	0	0	8	0	4	0	0	5	0	0	0	0	0	0	0	0	0	0	2	0	2	0	69	2	0	0	0	2	0	0	0	0	0	0	0	0	0	0	14	47	0	96	0	2	2	2	0	2	6	4	48	0	0	0	0	0	0	7	9	6	0	0	0	0	0	0	0	23	0	0
SP118	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_423	377	0	0	11	0	81	302	0	0	0	729	0	265	10	0	0	0	149	0	4025	0	0	0	0	0	486	438	1086	0	303	236	13	0	160	0	0	0	39	0	28	0	0	0	18	59	0	0	0	338	117	0	224	0	0	425	188	0	0	47	121	45	0	136	0	0	0	0	7219	1278	0	0	164	0	0	0	0	0	1209	0	187	0	0	0	214	0	0
SP119	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;denticariosi	4	0	0	0	0	0	0	49	0	0	0	147	0	0	25	381	0	0	0	7	1631	374	618	0	0	0	0	0	0	0	0	0	0	0	0	160	0	176	253	0	510	0	0	0	428	370	131	572	110	0	0	60	0	4	0	367	180	0	169	93	0	0	158	114	0	0	0	30	342	0	0	0	69	0	0	0	0	512	847	177	0	0	0	0	0	13
SP12	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;atypica	55	16	0	193	98	3	153	708	1703	539	0	1770	94	13	375	1737	693	37	1023	44	619	212	92	2	351	274	29	39	98	23	548	1152	1084	1389	2955	91	544	136	299	25	394	101	73	137	1412	1474	3685	229	499	453	645	179	1257	34	19	142	998	111	742	1129	1250	4494	115	932	167	45	71	932	2689	1226	2948	150	836	129	724	99	3243	3004	1069	941	14	0	121	374	43	47
SP120	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Megasphaera;micronuciformis	98	23	37	14	2	2	22	106	350	31	6	503	58	35	68	25	117	25	224	83	64	33	111	3	256	59	39	10	53	89	179	342	113	217	345	51	33	58	48	28	70	56	14	100	377	48	410	17	40	28	98	29	379	67	2	48	365	13	272	232	174	231	171	60	10	21	32	198	55	223	234	168	86	12	204	30	252	661	28	357	3	8	58	180	29	29
SP121	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_443	0	0	0	0	6	0	47	0	0	0	101	0	2	2	18	0	0	4	0	38	2	0	0	0	0	0	6	13	0	0	0	0	0	0	2	4	0	0	0	0	0	0	0	46	3	0	0	0	0	0	0	0	0	0	83	5	0	0	67	0	0	0	0	0	3	0	0	0	2	0	0	0	0	4	2684	0	0	0	0	0	0	158	5	0	0	0
SP122	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_169	16	0	0	0	52	6	12	0	0	10	12	46	62	0	23	140	14	0	6	20	5	14	0	0	32	74	0	9	19	5	0	8	21	5	0	45	44	0	0	165	0	0	0	17	20	2	9	18	11	0	3	0	7	0	2	9	3	108	0	24	0	11	3	6	57	6	107	5	22	7	12	2	2	45	0	8	0	0	23	163	7	0	2	13	15	0
SP123	Bacteria;Actinobacteria;Actinobacteria;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum	21	251	0	77	251	0	0	0	7	9	32	0	9	2	51	0	12	23	8	4	1	14	2	3	0	28	4	1	78	5	4	9	4	3	8	143	209	0	16	51	4	0	0	0	0	8	14	270	55	0	10	0	8	0	0	16	1	7	0	25	6	14	9	2	10	5	7	15	7	0	80	9	6	19	0	53	2	10	3	64	41	0	7	10	5	65
SP124	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Eikenella;corrodens	0	8	3	28	0	0	0	3	5	0	39	0	3	3	5	2	0	2	6	10	0	2	1	7	37	0	0	110	3	2	3	6	18	6	5	2	4	7	2	10	0	0	0	32	0	0	2	14	12	0	2	1	3	0	10	16	20	20	2	0	18	1	5	0	0	7	16	5	2	14	0	3	2	4	7	0	3	2	0	2	1	55	0	3	3	0
SP125	Bacteria;Saccharibacteria_(TM7);TM7_[C-1];TM7_[O-1];TM7_[F-1];TM7_[G-1];sp._oral_taxon_352	119	1234	100	50	11	67	502	0	401	299	95	925	75	80	198	3	63	168	190	193	19	36	113	198	66	116	117	38	411	62	52	130	14	108	153	87	119	51	769	423	3574	36	33	64	561	130	582	6	20	197	53	188	244	78	72	156	433	14	1011	51	126	577	99	28	310	25	14	66	65	70	542	73	30	229	6916	17	59	508	285	126	15	52	60	188	39	7
SP126	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;sputigena	2	5	3	0	56	0	0	10	0	8	0	0	0	1	0	0	42	0	61	0	2	0	4	0	1	0	0	0	0	0	3	6	0	31	21	0	5	0	13	0	0	0	0	0	0	2	0	7	51	2	0	0	0	0	3	0	2	18	6	61	64	22	0	2	3	0	8	22	0	55	8	2	2	4	0	0	0	88	2	5	0	0	0	6	0	0
SP127	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;sp._oral_taxon_917	0	0	0	0	0	0	4	26	0	303	0	788	0	0	0	8	3	0	83	0	0	0	42	0	0	84	2	0	0	0	0	18	2	0	4	0	0	0	0	0	0	30	8	9	2	0	1341	0	9	2	2	0	3	4	8	0	3	2	0	0	203	0	0	423	7	0	3	2	2	114	2	10	354	0	0	9	4	221	45	2	0	5	0	100	2	0
SP128	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_070	0	12	0	0	508	153	26	362	25	274	7	71	29	0	46	0	17	5	52	0	4	9	289	0	436	34	35	0	0	48	275	243	0	6	3	0	0	36	82	19	12	0	0	8	0	70	0	8	0	6	1	15	29	0	40	101	94	87	18	36	4	0	100	62	24	43	39	158	3	0	0	80	38	26	132	67	0	86	110	85	17	4	0	0	27	24
SP129	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;naeslundii	25	2	21	0	0	157	25	0	17	14	0	0	18	0	13	19	2	46	5	22	2	2	0	25	0	54	0	37	38	0	13	0	8	2	0	11	19	4	13	68	11	21	0	0	12	11	0	4	16	5	4	6	4	19	0	12	12	29	19	3	0	8	40	3	83	10	51	7	5	4	11	36	8	72	4	6	12	29	6	30	38	0	3	9	21	0
SP13	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;dentisani	1662	643	58	1345	2384	2688	5523	875	1305	1535	139	604	2837	12348	4851	591	1877	689	831	190	693	2646	1257	444	48	1293	13	157	290	1594	386	376	685	492	243	1270	338	195	3600	1179	323	81	58	202	54	1348	500	52	330	1051	249	442	154	950	616	561	73	2231	184	150	244	352	666	1096	796	235	977	723	711	3477	2680	745	2480	615	70	439	507	515	629	570	28842	248	101	1501	1183	58
SP130	Bacteria;Actinobacteria;Coriobacteriia;Coriobacteriales;Coriobacteriaceae;Atopobium;rimae	17	8	0	0	36	31	0	6	5	23	4	6	9	2	18	12	94	20	71	36	0	2	2	14	68	12	9	33	17	2	28	40	5	10	4	13	6	17	12	511	0	12	0	7	4	5	13	5	2	6	29	21	3	0	51	2	17	58	5	7	3	59	10	31	13	6	45	5	2	8	55	11	26	15	0	6	8	17	9	19	13	0	6	29	6	6
SP132	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;gingivalis	0	0	2	0	0	46	0	0	0	35	0	0	2	0	0	0	0	0	602	0	0	25	0	0	0	0	0	2	0	70	0	13	0	2	0	70	0	0	0	0	0	0	0	9	0	0	0	0	42	0	36	45	0	0	0	0	0	0	22	0	0	0	0	26	120	0	41	0	0	4	0	0	23	101	0	0	0	0	0	0	0	24	0	57	39	0
SP133	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;peroris	0	0	0	0	0	0	0	0	3	256	1	304	0	0	2	78	928	3	4	0	1	0	0	4	0	0	0	0	0	0	0	27	0	0	0	0	167	0	0	2	0	79	0	0	0	0	31	0	0	0	0	2	259	0	0	0	2	0	0	0	0	266	0	162	0	0	0	0	0	0	323	2	113	0	0	0	0	0	0	0	0	3	0	2	0	7
SP134	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;catoniae	0	148	9	0	0	20	8	0	43	2	0	0	24	118	4	0	2	49	0	0	0	0	48	20	0	0	0	0	69	0	6	0	0	0	0	97	17	8	32	0	0	0	0	8	0	0	0	131	0	19	33	84	0	0	0	38	1	43	0	0	9	2	11	0	49	5	35	0	0	6	15	15	2	41	48	6	0	96	3	0	28	53	0	10	2	0
SP135	Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Bergeyella;sp._oral_taxon_322	0	40	52	16	0	42	18	0	12	0	62	3	64	1	12	2	10	26	1	27	0	3	43	11	8	0	15	20	38	0	2	0	3	0	3	6	13	4	0	11	0	17	0	10	0	6	8	55	31	3	9	8	0	26	0	50	18	12	7	4	3	2	0	4	31	8	0	6	2	3	2	0	9	23	0	21	31	75	6	0	74	6	2	5	11	0
SP137	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;shahii	34	103	0	23	0	89	36	0	34	4	680	5	0	141	76	0	0	276	4	361	0	23	9	68	3	0	242	0	271	0	0	0	0	0	45	65	0	61	0	11	181	0	0	100	2	5	0	2	148	0	4	96	0	66	37	6	159	14	123	0	2	2	11	2	0	0	105	2	3	0	3	10	1	0	0	2	3	0	33	0	0	384	57	14	62	0
SP138	Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;gracilis	2	9	0	0	55	2	0	0	8	0	0	0	0	0	0	0	0	0	10	7	0	0	0	0	0	0	0	13	0	2	2	3	0	2	0	0	0	0	0	17	0	0	0	6	9	3	0	7	3	0	0	0	0	2	3	3	10	78	0	2	3	21	6	0	2	2	31	3	0	2	16	7	0	2	4	0	0	0	5	12	0	0	0	0	0	0
SP14	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;nanceiensis	29	150	86	129	29	270	2	0	41	4	72	5	193	199	30	0	14	213	2	77	163	28	202	151	103	107	37	492	170	359	4	12	176	74	112	271	9	107	105	38	34	486	17	93	183	126	9	180	347	678	3855	74	20	685	174	320	76	200	455	38	71	51	55	10	86	68	112	48	65	66	57	68	12	72	484	83	232	721	22	17	13	34	149	119	126	6
SP140	Bacteria;Proteobacteria;Betaproteobacteria;Burkholderiales;Burkholderiaceae;Lautropia;mirabilis	49	86	114	158	66	144	5	16	4091	62	21	8	157	99	380	1	15	84	112	131	18	25	246	207	11	143	8	156	105	2	2	7	12	0	3	4	101	6	20	106	32	35	27	34	197	36	110	325	109	140	63	25	8	61	15	136	59	715	119	35	20	170	56	10	116	25	359	44	69	21	147	71	27	94	96	27	92	48	39	25	43	7	3	48	17	4
SP141	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;gordonii	34	2	3	16	245	89	0	86	7	159	19	31	233	64	17	60	55	26	0	0	18	27	45	0	87	49	3	142	5	16	126	76	1	21	44	53	115	12	98	2	0	20	34	40	9	40	33	67	22	50	5	2	14	7	0	8	0	84	9	14	15	15	19	77	35	3	41	46	79	47	19	17	76	35	29	3	8	288	16	99	0	0	4	3	1	6
SP144	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;aurantiaca	0	180	0	4	0	0	7	0	7	0	108	3	0	26	0	0	0	165	0	0	2	0	26	448	0	3	659	18	214	0	0	0	0	0	0	2	0	0	0	0	3	16	0	23	0	0	0	0	215	0	2	0	5	246	282	0	0	0	0	0	0	0	0	3	0	0	0	0	2	0	0	0	3	0	0	0	0	0	3	0	13	598	34	13	0	0
SP145	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Oribacterium;parvum	0	3	0	20	0	0	14	0	0	0	0	0	0	0	0	0	0	0	0	87	0	0	0	0	0	0	0	0	2	0	2	0	0	0	0	3	0	0	0	0	112	5	0	0	0	0	0	0	9	0	0	0	0	0	0	0	69	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	26	51	0	5	0	12	0	0
SP147	Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Lactobacillus;gastricus	0	0	0	0	0	0	0	5	0	609	0	2	0	0	0	313	0	3	0	0	0	0	0	0	0	0	0	0	0	0	15	65	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	129	0	0	0	0	0	0	0	0	116	0	0	0	0	0	0	0	0	0	0	0	0	0
SP148	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteria_[O];Fusobacteria_[F];Fusobacteria_[G-1];sp._Oral_Taxon_A71	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	207	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	8	0	0	0	0	0	0	0	101	0	0	11	0	0	0	0	0	0	0	0	0	0	0	9	0	0	0	0	0	0	0	0	0	0	0	0	0	20	0	0	0	31	0
SP149	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;segnis	0	71	0	0	0	0	0	0	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	38	0	0	0	8	0	0	0	0	0	0	0	2	0	8	0	2	0	39	66	0	18	42	0	76	0	0	0	205	7	98	14	0	0	0	0	0	0	0	42	0	0	0	0	2	4	0	0	0	0	6	0	0	36	0	0	0	0	0
SP15	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Catonella;morbi	16	637	537	17	23	61	48	6	311	14	135	26	22	21	27	0	21	117	27	104	28	31	4	58	65	62	34	9	23	39	40	13	10	57	48	22	14	10	7	222	199	0	30	38	42	55	194	145	23	40	3	8	56	35	44	18	66	11	121	71	16	49	18	6	0	10	55	54	52	18	45	16	12	0	163	16	17	19	18	32	24	58	22	39	44	4
SP151	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Tannerella;forsythia	2	29	0	0	112	17	6	0	8	10	3	3	0	0	3	0	0	8	108	20	0	4	0	0	3	39	6	15	19	4	3	4	0	0	0	7	0	1	0	142	2	0	2	2	0	5	4	0	28	3	2	10	0	3	8	0	10	0	5	2	0	0	0	22	40	4	2	0	0	0	0	0	28	30	15	2	0	5	0	13	0	5	0	9	4	4
SP153	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;oralis	0	182	0	2	0	2	3	0	447	0	0	0	0	2	3	0	3	15	569	37	0	3	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	5	15	0	0	0	0	0	51	0	16	8	18	0	28	0	9	2	9	4	0	22	2	0	321	2	19	0	2	0	0	0	0	285	5	23	0	2	0	0	0	0	2	0	0	0	0	8	0
SP154	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;flava	0	0	0	0	0	0	0	0	71	0	0	0	0	10	0	0	0	58	17	17	0	239	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	14	3	264	0	0	0	265	0	0	0	0	3	0	0	0	0	0	26	0	23	0	0	3	0	0	0	50	0	16	0	0	0	3	0	0	0	0	3	5	0	0	0	0
SP157	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;oligofermentans	0	171	0	0	0	0	110	0	2	0	0	0	0	5	0	0	0	50	0	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	10	0	0	0
SP159	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;hongkongensis	9	13	0	0	127	11	0	2	13	2	0	0	28	0	0	54	25	44	16	10	7	0	7	0	0	0	0	15	6	10	3	0	1	0	0	4	22	0	22	121	2	0	0	0	8	76	30	14	0	0	0	6	0	0	3	0	0	1	5	4	26	20	0	5	4	0	15	4	12	17	14	3	2	9	0	2	0	5	12	86	0	0	2	0	2	2
SP16	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;graevenitzii	594	39	66	33	2	5	64	776	446	1233	295	1968	366	135	133	12	545	1331	2268	689	116	393	502	283	1084	300	40	288	35	376	1456	1292	4	879	94	103	695	134	611	906	1718	81	93	320	1098	177	2257	3	11	132	312	48	1007	51	71	0	39	51	932	219	141	559	338	601	50	102	90	257	387	145	452	327	650	38	345	141	320	607	540	652	4	163	529	348	35	562
SP162	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;sicca	0	21	0	12	0	0	4	0	9	0	0	0	37	0	0	172	0	0	37	0	0	0	0	0	0	0	0	0	9	2	2	27	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	691	0	0	0	0	0	0	8	0	0	767	0	1	6	0	10	0	0	0	377	0	0	0	0	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SP163	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp._oral_taxon_914	0	0	0	0	0	0	0	0	0	0	0	0	24	7	0	0	0	0	0	0	0	0	0	0	83	0	0	3	0	0	2	0	0	4	0	0	0	2	0	0	0	14	0	0	0	0	0	0	184	180	1	0	0	0	144	68	13	0	56	0	0	0	0	0	15	0	31	0	0	0	0	0	0	9	143	0	0	9	0	0	0	0	0	0	5	0
SP164	Bacteria;Firmicutes;Clostridia;Clostridiales;Ruminococcaceae;Ruminococcaceae_[G-1];sp._oral_taxon_075	4	3434	8	16	506	9	180	20	198	63	31	10	264	12	486	3	34	391	71	338	26	15	86	42	2	114	26	51	106	2	13	64	3	8	43	99	0	34	52	63	109	12	0	11	284	67	68	57	22	17	7	14	5	10	25	27	18	2	2271	2	19	611	8	13	15	66	4	9	16	14	478	14	19	10	5776	68	6	301	0	9	16	7	13	6	10	9
SP165	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp._oral_taxon_458	0	36	4	0	0	0	0	3	11	0	0	0	0	2	1	0	0	10	0	2	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	42	0	54	77	0	2	0	38	0	7	4	7	3	240	0	2	0	0	0	0	0	0	106	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	2	0
SP167	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;sp._oral_taxon_892	0	188	0	0	0	0	0	0	8	7	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	16	0	0	2	0	0	4	0	0	0	0	67	0	0	0	0	0	0	0	27	0	0	0	0	0	0	450	3	0	21	0	0	0	0	0	0	0	0
SP168	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Bacteroidales_[F-2];Bacteroidales_[G-2];sp._oral_taxon_274	0	1	0	0	62	0	0	0	0	5	25	0	0	7	3	0	0	0	72	123	0	6	39	36	5	97	0	4	5	4	6	2	0	0	0	9	0	0	0	97	0	1	0	14	0	0	0	2	10	0	0	0	0	5	8	2	0	0	0	3	0	0	0	15	0	4	0	0	0	0	0	0	21	0	80	9	0	0	0	21	5	0	0	7	3	0
SP169	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;nucleatum_subsp._animalis	3	30	0	0	193	3	2	27	14	2	1	11	10	6	18	10	22	0	21	151	4	6	51	8	17	14	2	40	4	9	1	9	8	8	6	5	6	2	3	358	0	0	0	0	5	17	29	7	11	3	1	8	0	6	12	6	6	159	3	4	2	17	11	10	0	7	81	12	12	6	18	14	19	3	2	6	0	16	9	55	2	4	0	6	2	4
SP17	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._oral_taxon_279	1004	4373	19373	2005	274	438	612	125	118	99	6052	183	2409	3395	280	22	345	3049	83	3314	553	1237	3069	10062	288	543	754	2762	1242	9434	180	74	615	1553	1416	4128	393	294	8472	141	4952	243	74	480	187	1169	93	1953	6989	1735	3898	1824	261	3209	4492	5150	3430	970	789	281	444	272	220	211	1659	208	1799	1101	95	383	668	202	278	1170	1509	308	2334	626	833	174	1063	3529	212	1033	392	27
SP170	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;pharyngis	0	0	0	0	213	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	344	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0
SP173	Bacteria;SR1;SR1_[C-1];SR1_[O-1];SR1_[F-1];SR1_[G-1];sp._oral_taxon_875	0	1700	37	51	0	17	36	0	8	2	256	0	15	231	92	0	4	103	30	360	6	30	3	210	4	1	211	18	822	2	0	0	2	3	12	0	3	161	2	94	4	47	0	41	3	2	6	21	14	64	90	7	6	54	170	52	437	48	579	3	10	0	4	2	0	169	487	138	0	15	53	4	19	0	320	250	2	403	2	0	345	14	32	61	9	0
SP174	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;dianae	0	50	0	0	25	0	190	0	0	0	0	0	0	3	0	0	0	0	49	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	425	0	12	0	5	0	2	0	12	0	0	93	90	11	28	0	0	0	0	0	28	0	0	24	3	2	0	8	0	0	0	2	0	0	0	0	0	0	0
SP175	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;sp._oral_taxon_203	0	12	2	0	173	2	0	0	0	0	0	0	0	0	0	0	2	13	36	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	199	0	0	0	13	12	0	0	0	0	5	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	9	0	0	0	0	0	0	2	0	0	6	0
SP176	Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sputigena	15	139	5	16	3	13	14	0	5	15	7	0	23	22	5	0	1	8	11	14	3	0	31	292	0	5	5	270	14	0	0	0	7	2	8	0	31	0	148	4	10	0	0	22	0	19	3	7	19	5	3	6	3	2	31	20	49	56	9	0	11	9	3	16	3	9	50	0	4	16	10	0	14	3	142	14	6	18	2	29	13	33	0	4	15	1
SP177	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_309	0	0	0	0	0	0	2	1	37	17	0	113	0	0	37	0	7	4	48	16	0	7	9	2	0	15	5	0	0	0	6	286	0	22	36	7	0	1	9	10	0	18	9	12	52	29	57	0	22	10	20	3	2	0	0	5	0	0	23	0	55	51	6	4	0	2	4	0	0	48	24	8	11	0	311	0	3	231	10	0	0	0	2	36	0	0
SP178	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_498	0	0	0	0	0	0	6	3	8	0	0	0	0	0	6	0	2	0	22	441	2	0	2	10	19	0	0	0	0	0	0	5	4	0	0	0	0	0	42	0	0	21	0	0	2	24	0	0	5	0	3	0	0	0	0	0	10	0	0	2	13	13	0	0	2	0	0	0	0	8	12	0	1	4	0	2	0	30	1	0	0	0	2	0	0	0
SP18	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;flavescens\|subflava	174	7282	64	910	90	174	2	29	918	15	381	38	2579	2825	59	12	18	819	1033	689	1089	2516	234	1558	25	294	461	9312	792	45	34	52	80	180	10	30	1766	87	484	200	1585	96	20	99	916	990	103	13793	2947	383	244	6411	25	1800	4686	2788	2957	1343	2044	270	289	143	225	25	172	581	722	583	196	371	168	205	44	142	222	747	520	51	28	340	218	68	17	131	227	14
SP180	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;infelix	0	125	0	0	144	0	0	0	0	2	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	13	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	114	0	0	0	0	0	0	0	0	0	7	0	0	60	0	0	0	0	0	0	0	0	32	0	0	0	0	152	8	0	1	0	0	0	4	0	2	0	0
SP181	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp._oral_taxon_513	0	76	0	0	0	0	2	0	0	0	0	0	0	0	2	0	0	3	146	2	0	0	0	0	0	1	7	0	0	0	0	0	0	0	3	0	0	0	4	0	0	0	0	0	0	0	2	4	0	0	0	76	0	0	7	13	0	825	27	0	29	0	13	2	3	0	333	0	0	20	0	16	2	0	201	0	0	0	0	0	4	0	0	0	0	0
SP183	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_057	344	0	42	25	11	103	2	157	256	100	2	33	68	23	23	726	34	60	60	162	44	27	122	30	157	7	15	11	62	609	548	69	18	99	23	455	162	25	150	182	0	25	89	134	144	115	342	4	35	111	85	14	506	13	3	31	34	34	10	184	16	0	108	108	80	39	25	119	186	14	7	102	119	68	5	193	101	254	176	147	4	40	29	601	86	25
SP185	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;buccalis	0	0	0	0	0	0	6	0	100	2	0	0	12	3	2	0	3	5	4	3	8	2	0	3	5	6	0	0	2	5	2	3	0	0	8	8	2	4	0	4	0	0	0	0	7	30	22	0	1	0	4	10	0	4	4	6	16	61	6	0	0	9	7	0	0	2	31	0	0	0	5	3	2	0	23	0	1	9	3	0	0	0	0	0	0	0
SP186	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;rava	10	2	0	0	0	0	10	6	6	5	2	0	18	0	28	0	0	5	251	0	0	23	9	0	0	0	18	0	0	0	21	1	0	0	7	4	0	14	0	5	0	34	0	29	10	0	2	0	179	29	59	27	1	78	7	21	346	2	260	2	394	5	0	0	0	2	0	0	0	469	13	0	2	0	2	0	309	0	10	0	2	0	19	22	12	0
SP187	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_170	0	0	0	0	2	0	0	0	0	0	0	0	0	0	2	0	2	0	0	0	6	16	22	0	2	0	0	0	0	7	0	6	0	0	0	0	0	0	0	96	0	0	0	0	29	5	0	8	0	0	0	0	3	8	2	0	2	0	0	10	0	0	5	2	0	9	2	6	18	5	0	0	0	0	0	5	0	4	28	51	0	0	0	0	6	4
SP188	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;veroralis	0	0	0	0	23	0	3	0	0	0	0	0	0	3	0	0	2	9	0	20	0	0	0	65	27	0	6	0	0	0	5	0	0	2	0	0	0	0	0	5	0	0	0	0	6	0	0	0	31	0	0	0	2	0	0	0	0	479	0	0	0	131	2	0	6	0	219	3	0	0	79	0	0	0	0	0	0	0	0	0	11	4	0	0	2	0
SP189	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;micans	18	59	0	0	49	0	4	0	6	2	20	0	2	7	0	0	0	8	8	13	0	0	0	2	0	4	4	12	5	0	6	2	0	0	10	0	0	0	0	0	0	0	0	28	0	0	0	0	0	0	0	0	0	2	0	0	11	33	0	0	17	0	2	5	0	0	18	0	2	6	0	0	2	0	0	0	0	4	0	0	0	9	0	0	0	0
SP19	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Lachnoanaerobaculum;umeaense	107	0	28	21	8	5	134	31	51	2	142	0	101	32	62	0	22	143	13	65	9	28	83	168	28	10	100	15	30	89	0	57	2	20	24	25	24	18	53	159	204	21	0	57	10	16	0	13	25	16	18	11	14	70	5	22	51	25	17	11	5	22	4	0	77	29	82	6	22	16	11	2	2	80	115	40	55	3	29	12	10	57	17	28	23	3
SP190	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_074	44	2	52	193	836	352	0	167	294	13	150	0	43	6	51	3	338	148	495	243	420	273	161	29	1937	75	81	384	6	438	659	87	4	445	1	28	4	206	222	5	328	18	39	70	112	349	11	124	157	4	52	481	12	0	0	75	0	218	100	177	104	12	422	2	592	145	205	131	215	160	8	524	16	437	61	183	0	69	197	3	2	13	6	189	0	73
SP193	Bacteria;Proteobacteria;Gammaproteobacteria;Cardiobacteriales;Cardiobacteriaceae;Cardiobacterium;hominis	7	49	0	39	22	35	2	0	4	4	1	0	22	3	3	0	0	10	4	24	1	0	48	8	0	4	2	14	7	0	7	0	3	0	2	12	22	0	2	77	7	0	0	0	7	4	3	11	30	4	0	6	4	5	6	11	9	48	0	10	6	6	9	2	0	2	16	4	11	0	8	8	2	2	36	5	0	10	2	20	10	3	0	2	0	0
SP196	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sinensis	18	47	0	0	0	0	0	0	0	4	2	0	0	5	0	0	0	0	20	0	0	4	0	0	0	0	3	0	0	174	11	0	0	0	0	0	0	19	0	16	2	0	0	6	0	0	0	0	67	14	0	0	0	0	5	0	25	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	49	0	0	0	12	0	0	2	0
SP197	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Lachnospiraceae_[G-3];sp._oral_taxon_100	0	21	0	3	0	4	7	0	0	0	233	2	14	14	3	0	0	14	0	22	0	0	0	133	0	0	12	20	15	0	0	0	0	0	0	0	2	0	0	24	153	0	0	14	0	3	0	0	0	0	0	0	2	0	11	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	308	0	0	0	0	0	3	145	23	0	0	0
SP199	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;intermedius	20	3	19	0	231	4	6	6	27	8	27	2	3	1	28	19	7	24	0	4	3	2	0	8	42	29	2	92	28	0	0	13	2	7	4	8	60	3	7	100	1	0	0	6	16	55	23	6	38	17	4	5	0	12	1	9	12	135	0	0	6	20	23	11	33	3	83	12	8	10	13	20	11	27	3	3	23	19	24	22	18	0	0	75	4	8
SP2	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcaceae_[XI][G-1];[Eubacterium]_sulci	65	21	385	38	10	11	79	16	56	33	49	3	190	50	79	4	13	213	9	138	18	71	215	26	11	49	245	27	148	343	9	6	0	57	96	64	6	157	7	434	568	76	12	181	54	84	99	16	49	115	419	133	147	78	115	23	219	12	345	177	59	70	192	2	115	81	82	145	53	69	58	204	5	101	0	87	187	90	178	568	4	171	120	373	101	54
SP20	Bacteria;Actinobacteria;Actinobacteria;Propionibacteriales;Propionibacteriaceae;Propionibacterium;propionicum	0	18	0	13	3	8	7	0	14	7	2	0	37	36	0	0	6	26	18	21	0	6	28	49	4	0	2	36	30	19	7	3	3	4	6	14	11	6	3	29	0	0	0	14	0	4	0	44	16	5	0	20	6	18	8	8	2	21	26	7	0	80	44	2	9	0	11	2	0	5	38	44	5	11	0	0	12	22	10	4	17	9	0	2	7	0
SP200	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_305	8	0	0	0	0	0	0	0	11	23	0	9	0	0	0	0	2	0	27	0	13	0	5	0	0	3	0	2	0	0	4	2	12	3	4	4	4	2	0	4	0	13	0	13	61	20	12	0	7	0	24	3	8	0	0	7	0	0	0	0	30	16	3	0	0	0	0	0	5	30	23	2	5	0	0	3	9	36	15	10	0	0	0	29	0	8
SP203	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp._oral_taxon_308	706	10	9	10	24	3	113	11	68	13	6	182	32	0	190	4	19	14	20	7	65	22	56	14	386	49	108	108	3	5	27	23	62	29	24	322	11	80	47	3	0	155	26	73	456	80	100	14	48	1392	248	374	89	11	58	251	92	98	565	152	165	110	933	7	24	105	305	114	288	192	93	880	21	27	20	113	109	101	717	31	8	5	10	51	187	63
SP206	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;oris	0	0	2	0	32	0	2	0	0	5	2	0	57	0	6	0	6	26	0	0	0	3	0	2	2	3	0	0	7	8	24	2	0	0	7	0	11	0	9	44	4	0	0	34	9	7	2	5	3	0	0	0	0	0	0	3	3	4	0	0	4	0	6	5	0	0	0	3	8	0	0	8	1	0	0	2	2	10	9	44	9	0	10	0	0	20
SP21	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_218	12	2	0	0	0	0	110	49	288	1	0	0	9	0	15	0	0	717	220	208	0	53	33	12	56	0	425	0	0	0	30	17	0	30	0	0	0	2	0	11	2	5	22	40	6	47	0	0	21	78	37	22	3	0	3	0	0	0	6	0	84	523	0	11	0	0	0	0	0	63	372	0	24	0	0	2	24	0	22	3	0	0	0	127	121	0
SP210	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Micrococcaceae;Rothia;aeria	69	916	68	978	0	772	306	69	491	351	217	0	150	212	434	176	0	152	184	217	2	98	63	51	9	527	45	38	218	4	0	18	152	14	47	8	380	15	252	64	101	11	1	64	46	73	37	384	167	20	56	132	33	67	6	163	68	751	133	144	292	116	55	9	64	78	414	56	52	97	629	109	74	49	148	302	123	74	41	15	507	7	47	87	69	63
SP214	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;mutans	0	0	0	0	0	0	0	6	11	13	0	0	0	0	0	36	0	0	111	0	3	0	6	0	0	2	0	0	0	0	22	39	0	0	0	0	0	0	0	5	0	0	0	22	7	2	0	7	3	3	0	0	0	0	0	0	2	0	0	0	0	0	0	4	6	0	0	0	0	0	0	0	2	4	0	0	0	35	0	4	0	0	0	0	0	3
SP215	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;hofstadii	0	0	0	0	1	0	0	0	11	0	0	0	4	2	0	0	0	14	0	10	6	3	2	3	0	42	6	0	24	0	5	3	3	2	4	17	13	2	0	0	1	0	0	0	0	13	3	1	3	2	0	0	0	5	0	0	3	49	7	12	0	0	0	0	0	0	24	5	2	13	0	0	5	0	0	0	0	14	6	23	0	0	0	8	0	0
SP217	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._oral_taxon_285	24	386	0	0	0	0	33	0	0	0	343	2	0	0	11	0	0	30	0	300	0	0	0	23	2	0	579	50	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	75	0	0	0	0	0	0	0	52	0	0	2	0	0	0	160	0	0	0	0	2	0	0	6	0	4	0	0	2	5	0	120	0	0	0	0	0	0	478	0	0	0	0
SP22	Bacteria;Firmicutes;Bacilli;Lactobacillales;Aerococcaceae;Abiotrophia;defectiva	6	74	107	116	0	2	148	180	105	630	157	9	20	60	11	10	11	467	54	10	13	292	56	3	8	30	36	1358	417	2	14	35	22	8	133	82	78	2	223	0	2	14	0	21	0	91	0	173	31	593	30	0	4	20	15	43	5	168	54	5	466	13	207	242	59	67	75	129	7	96	30	201	232	53	2	141	12	335	0	2	168	47	6	10	9	23
SP227	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcaceae_[XI][G-7];[Eubacterium]_yurii_subsp._yurii_&_margaretiae	0	376	4	0	0	0	6	3	0	7	28	0	7	0	0	0	0	5	0	0	0	0	6	0	10	2	0	0	3	0	0	0	2	0	0	0	0	4	0	20	0	0	0	0	0	2	2	0	0	0	0	7	0	3	2	17	0	0	4	6	0	6	4	5	7	0	4	4	0	0	6	3	2	7	104	0	0	7	0	12	7	0	0	3	0	0
SP229	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;sp._oral_taxon_036	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	2	0	2	209	0	0	0	0	0	0	0	0	0	0	652	0	0	0	7	0	17	0	2	319	0	0	6	0	0	17	0	0	0	2	0	0	0	0	0	0	0	0	0
SP23	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;anginosus	101	5	0	0	244	15	4	66	9	26	0	8	5	6	0	12	59	6	13	16	2	14	11	16	106	17	11	53	61	26	19	29	23	11	8	4	8	12	25	33	4	25	0	3	3	49	26	2	83	16	14	16	0	0	7	12	2	111	18	3	0	0	24	22	10	4	63	16	19	5	0	26	13	11	15	5	0	32	24	92	15	0	3	21	3	12
SP233	Bacteria;Saccharibacteria_(TM7);TM7_[C-1];TM7_[O-1];TM7_[F-1];TM7_[G-4];sp._oral_taxon_355	0	30	0	0	2	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	9	0	0	0	13	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	491	0	0	0	0	0	8	0	2	0	0	0
SP235	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;sp._oral_taxon_018	0	0	0	2	0	0	0	0	11	0	0	0	0	6	0	0	0	2	3	26	0	0	0	0	0	0	0	0	0	0	0	3	8	3	0	0	0	0	0	0	0	0	0	0	0	15	0	183	30	2	0	35	0	0	0	2	2	0	0	0	29	0	3	0	0	4	0	0	0	34	0	5	0	0	0	0	0	5	0	0	0	0	0	0	0	0
SP239	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Tannerella;sp._oral_taxon_286	49	0	0	0	0	0	14	0	1	0	184	2	12	3	49	0	0	76	2	130	0	3	0	67	53	0	1	0	0	2	4	0	0	0	0	20	2	4	55	29	0	3	0	42	75	2	0	2	0	0	0	2	0	4	0	0	2	28	35	0	0	0	0	0	0	10	8	0	0	2	2	0	2	0	5	24	0	0	0	0	0	104	3	0	2	0
SP24	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Butyrivibrio;sp._oral_taxon_455	38	5	3	0	0	0	25	4	56	19	0	4	0	0	0	0	2	9	22	34	0	4	16	0	0	2	2	0	3	10	0	3	3	2	0	0	2	0	0	75	2	0	0	17	18	5	30	0	10	3	3	0	5	0	2	0	52	0	60	3	10	4	0	6	0	0	0	2	0	6	3	0	5	0	0	0	26	7	55	107	0	0	5	27	3	0
SP240	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcaceae_[XI][G-9];[Eubacterium]_brachy	3	66	3	0	15	0	2	3	9	9	11	0	7	3	16	0	0	41	93	14	0	6	2	0	0	11	0	26	20	0	3	0	0	4	0	0	0	11	3	110	11	0	0	6	5	6	8	2	40	4	0	2	0	0	6	0	4	13	7	3	0	0	2	0	6	2	10	0	0	0	2	0	0	5	0	5	2	6	4	16	6	0	0	19	0	3
SP243	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._Oral_Taxon_B43	0	119	0	26	0	0	0	0	0	0	0	0	0	0	0	0	0	31	0	0	0	0	0	16	0	0	0	0	0	0	0	0	0	17	0	0	25	0	0	0	0	0	0	9	0	0	0	0	0	0	0	0	0	0	17	1	5	28	0	0	1	0	0	0	0	0	14	0	0	3	0	0	0	0	0	0	5	0	0	0	73	0	0	0	35	0
SP247	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_056	44	2	0	31	9	220	18	0	10	17	19	0	87	23	6	0	0	5	0	28	3	47	43	16	0	11	2	23	18	0	0	9	8	4	2	29	5	3	0	91	0	0	0	2	0	10	2	59	22	0	0	5	12	0	0	17	6	0	17	6	8	0	2	3	3	6	14	9	0	2	5	8	5	0	0	14	6	32	0	10	107	3	0	12	0	0
SP25	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;sp._oral_taxon_136	10	0	2	0	25	0	9	19	116	24	2	47	0	2	15	0	0	2	24	79	2	3	0	0	5	5	1	0	0	0	0	64	0	0	254	4	8	2	5	3	14	0	0	11	34	2	78	2	0	28	8	0	2	50	10	18	395	5	501	93	118	142	8	48	7	0	0	40	8	63	94	8	40	7	101	9	39	241	48	0	0	0	21	0	7	2
SP251	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcaceae_[XI][G-7];sp._oral_taxon_922	0	166	0	0	0	4	0	0	12	0	45	0	0	0	0	0	0	35	0	41	0	0	4	27	0	0	1	0	17	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7	0	0	6	0	0	0	0	0	0	0	0	0	8	0	0	0	0	0	0	0	200	0	2	0	0	0	0	8	0	3	0	0
SP252	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._Oral_Taxon_B66	0	0	0	4	0	0	0	1142	4	0	0	0	0	0	0	88	0	40	36	0	0	5	0	12	0	0	9	0	0	21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	58	0	0	0	75	8	1	0	6	0	0	0	59	0	0	0	0	3	0	0	2	0	0	0	0	0	0	0	0	0	0
SP255	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Mogibacterium;diversum	657	43	40	26	0	1	167	172	205	81	245	197	33	27	28	0	58	317	118	96	15	99	102	59	49	29	233	161	34	342	133	109	100	119	134	235	24	42	8	444	137	25	23	241	80	53	99	10	43	63	158	35	431	21	97	28	59	0	57	129	94	103	128	31	91	69	180	63	87	56	158	137	69	74	19	169	121	61	282	196	2	53	169	536	245	211
SP257	Bacteria;Actinobacteria;Actinobacteria;Bifidobacteriales;Bifidobacteriaceae;Scardovia;wiggsiae	2	0	0	2	3	2	0	0	0	20	0	0	0	0	3	2	5	0	0	6	0	2	3	0	19	0	0	3	0	5	0	0	7	0	0	0	4	0	0	4	0	0	0	3	0	0	0	2	0	0	0	0	0	0	0	15	3	51	0	2	0	0	3	9	63	0	21	5	0	7	2	7	6	49	0	4	0	2	0	0	0	0	0	10	0	2
SP259	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptoniphilaceae;Parvimonas;micra	33	76	2	0	85	7	3	52	10	54	0	5	16	88	51	4	66	176	451	34	3	9	0	0	2	26	7	246	83	327	52	13	4	18	0	31	23	14	4	178	17	0	0	6	24	84	37	4	324	43	10	4	2	10	4	12	3	64	25	0	4	14	20	22	63	17	30	2	5	31	14	22	9	42	8	15	6	19	47	23	15	3	7	93	17	65
SP26	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;lingnae_[NVP]	227	37	367	134	40	156	226	150	97	425	80	1559	71	72	67	62	289	87	138	128	146	31	123	50	1317	79	21	94	53	361	196	139	74	378	110	166	222	45	340	539	288	47	25	125	95	77	432	55	139	50	192	27	183	76	104	28	77	76	106	314	88	488	303	59	130	27	144	231	94	99	365	356	49	100	77	34	128	58	258	292	27	35	149	294	76	215
SP264	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcaceae_[XI][G-5];[Eubacterium]_saphenum	0	0	0	0	16	0	0	0	6	102	4	0	0	5	0	2	0	34	284	0	0	12	0	0	0	13	0	0	0	28	0	0	0	0	0	0	0	4	0	9	0	0	0	0	6	0	0	0	179	0	2	29	0	2	0	0	2	0	7	0	0	0	0	3	25	0	0	0	0	0	0	0	2	26	0	0	0	0	0	0	56	0	0	0	11	0
SP266	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_304	0	0	6	0	0	0	0	14	0	5	0	0	5	17	0	0	0	0	563	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	2	13	0	5	0	0	0	0	56	0	5	30	7	2	0	0	0	0	0	0	0	0	0	0	70	0	6	0	0	2	0	0	0	55	0	0	0	0	0	0	28	0	0	2	32	0
SP269	Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Erysipelotrichaceae_[G-1];sp._oral_taxon_905	3	160	0	0	0	3	0	0	2	6	5	5	2	0	2	0	2	8	0	3	0	2	0	0	0	0	0	26	3	0	3	2	0	0	0	2	0	0	0	75	5	0	0	0	0	0	4	19	0	0	2	2	0	0	2	0	0	12	5	0	0	2	0	0	0	2	10	0	2	0	0	0	0	0	34	2	0	4	0	0	0	2	0	0	0	0
SP27	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_215	445	19	343	59	17	0	57	6	272	4	196	34	222	157	174	4	249	570	24	421	180	37	64	526	1006	71	58	223	39	354	7	36	29	362	156	283	58	37	362	508	446	34	11	136	58	51	41	16	118	16	48	44	25	20	121	11	72	56	19	352	118	149	45	3	8	53	52	159	80	86	106	34	55	6	523	82	24	91	117	113	5	98	170	60	49	6
SP277	Bacteria;SR1;SR1_[C-1];SR1_[O-1];SR1_[F-1];SR1_[G-1];sp._oral_taxon_345	4	369	4	0	0	9	15	0	12	0	21	0	91	10	128	0	0	96	10	386	4	0	0	42	4	0	74	25	25	0	0	2	0	3	0	3	2	18	0	1166	545	4	2	43	0	2	0	43	10	3	4	1	3	7	35	14	229	0	1743	4	0	4	4	2	0	0	14	0	0	0	7	4	2	0	2392	22	0	57	4	0	19	427	0	0	0	0
SP279	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;artemidis	2	0	0	0	416	0	0	0	63	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SP28	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_180	438	3017	319	466	65	568	986	541	122	653	1093	12	2068	1291	245	25	49	1520	121	1400	802	184	95	2313	242	529	707	803	918	2471	49	269	85	1646	984	1367	12	654	1509	1511	1877	48	20	385	2980	613	1322	358	535	49	416	615	405	449	249	56	201	289	696	380	195	11	649	28	834	390	1253	876	954	217	25	622	553	620	1115	460	833	78	1595	369	26	340	374	26	629	156
SP280	Bacteria;Actinobacteria;Coriobacteriia;Coriobacteriales;Atopobiaceae;Olsenella;sp._oral_taxon_807	0	2	0	3	0	11	0	0	4	16	0	0	0	9	2	0	21	0	16	9	3	2	0	2	2	0	0	0	0	0	8	7	0	6	3	0	0	2	0	30	0	0	3	0	3	0	4	0	4	0	3	4	4	0	20	0	19	12	0	0	0	71	4	0	7	0	15	0	0	0	59	8	6	5	0	0	0	2	8	19	0	4	0	10	5	0
SP282	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_171	13	2	0	3	23	5	2	0	9	0	15	0	0	0	0	0	5	11	0	4	2	0	2	0	0	4	0	0	0	0	0	0	0	0	0	39	15	0	0	133	4	0	0	0	3	7	4	35	16	0	0	0	3	2	0	33	0	6	0	13	0	0	0	0	16	0	7	6	15	0	3	0	0	12	0	0	6	5	0	53	0	0	11	0	4	4
SP284	Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;fastidiosum	0	209	0	0	65	0	0	0	0	0	0	0	0	6	0	0	0	0	45	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	3	10	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	3
SP29	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;scopos	0	2	186	0	0	0	0	1	390	0	0	0	0	39	0	0	7	0	0	0	0	6	0	81	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5	0	0	0	0	0	0	0	0	27	0	6	0	0	0	0	0	0	0	65	0	0	0	0	0	0	0	0	53	0	0	0	0	0	0	17	0	0	0	0	0	0	0	0	0
SP291	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Mogibacterium;timidum	0	0	0	0	6	0	0	19	0	0	11	0	6	0	4	2	0	18	72	0	0	20	0	0	4	3	4	0	21	18	18	0	0	2	0	15	0	0	0	91	0	0	0	0	0	13	6	4	5	2	6	0	0	0	0	0	0	0	0	0	0	0	4	3	23	0	0	0	0	3	0	2	3	16	0	0	0	13	0	2	34	0	0	30	0	0
SP293	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;pleuritidis	0	9	0	0	0	0	0	0	10	2	0	0	0	0	0	0	0	0	157	0	0	2	0	0	6	7	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	33	0	0	0	0	4	0	0	0	35	0	0	0	21	0	5	0	0	32	0	3	0	16	5	3	0	4	0	0	4	0	0	0	0	0	0	0	0
SP298	Bacteria;SR1;SR1_[C-1];SR1_[O-1];SR1_[F-1];SR1_[G-1];sp._oral_taxon_874	0	108	2	8	156	7	8	5	14	6	27	0	55	13	7	0	0	16	0	140	11	12	0	40	0	2	0	43	55	0	0	8	0	0	11	12	0	3	0	9	0	0	0	0	0	17	9	2	39	8	2	2	0	2	14	30	22	0	31	0	0	7	11	6	4	2	0	0	0	0	6	11	5	0	978	0	0	26	0	0	0	0	3	12	7	0
SP30	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;oralis	713	6	0	0	567	181	0	170	0	215	0	263	568	0	13	0	497	0	54	5	0	106	1	0	0	531	57	0	667	0	0	18	3	0	92	1032	113	1	29	0	0	0	7	33	0	19	99	147	0	38	0	283	29	173	89	43	0	0	42	11	158	199	80	177	224	0	0	20	0	52	130	98	118	147	0	0	89	0	0	0	141	14	43	0	0	4
SP300	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Dialister;pneumosintes	0	24	0	0	106	0	0	0	9	0	0	0	0	0	2	0	0	5	53	0	0	0	0	0	0	0	0	34	0	0	0	4	0	0	0	0	0	0	0	4	0	0	0	0	0	0	8	7	40	3	0	0	0	0	20	12	0	0	3	0	0	11	0	0	0	0	0	0	0	3	5	0	3	0	14	0	0	3	0	0	0	0	0	0	0	2
SP301	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Micrococcaceae;Rothia;dentocariosa	67	0	0	134	3774	36	135	25	55	153	247	365	985	19	105	182	341	130	95	48	193	175	64	11	85	193	6	196	0	35	509	398	113	101	26	472	764	24	126	306	100	43	29	76	12	236	149	335	21	39	68	75	100	26	36	127	25	370	19	54	228	136	73	84	409	55	195	131	170	122	295	113	98	327	5	360	38	87	27	266	51	18	64	13	48	166
SP302	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_448	0	2	3	5	356	18	0	7	7	4	0	21	2	0	8	2	21	18	53	4	6	3	17	5	166	8	0	9	2	4	22	17	0	13	0	5	4	0	0	92	6	0	0	0	13	14	4	105	25	0	0	9	9	0	0	17	3	20	5	0	0	35	0	8	61	0	6	6	2	24	21	0	4	65	0	0	12	26	110	19	0	0	0	0	10	9
SP31	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Oribacterium;sinus	75	161	477	71	432	85	73	56	412	54	102	88	31	41	64	4	135	149	63	317	37	37	73	141	187	171	54	56	44	59	172	107	70	252	88	27	165	26	20	133	299	14	24	37	65	47	889	941	11	109	59	20	193	37	15	21	72	18	126	113	51	102	31	22	41	13	88	79	46	55	74	30	36	25	294	28	83	118	128	181	31	61	25	94	39	96
SP311	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;johnsonii	15	6	0	0	8	6	0	14	9	4	8	0	19	0	4	0	0	11	7	10	4	4	4	9	8	6	2	72	0	3	0	2	0	2	0	0	4	0	15	32	0	0	0	13	3	5	0	2	7	0	0	0	0	7	2	6	5	3	0	7	0	2	0	3	0	0	11	2	2	5	8	0	0	0	5	0	0	0	6	12	0	0	0	5	5	2
SP315	Bacteria;Actinobacteria;Actinobacteria;Corynebacteriales;Corynebacteriaceae;Corynebacterium;argentoratense	0	0	7	327	0	0	0	2	0	0	5	0	0	0	0	0	0	0	7	0	390	115	0	0	0	5	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	4	0	0	0	0	0	0	0	0	0	0	0	8	0	0	0	3	6	4	6	2	1592	3	0	0	0	3	0	6	228	1688	2	5	0	5	0	0	0	0	6	0	0	0	0	0
SP319	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;mucosa	3	2	0	0	0	0	0	23	0	0	3	0	0	35	0	56	0	0	0	0	3	0	0	0	0	2	4	691	0	1	0	0	14	0	2	0	8	3	2	0	2	0	0	0	0	0	0	0	117	0	0	0	0	46	0	373	0	0	0	7	0	62	0	4	5	0	0	0	32	0	56	0	16	0	4	0	0	9	0	0	0	1	0	0	6	0
SP32	Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;showae	0	693	0	0	40	0	0	3	2	0	47	0	0	5	5	0	0	11	0	2	0	0	0	8	7	0	0	20	0	0	0	0	3	0	0	0	0	2	0	0	0	0	0	0	0	0	0	5	0	0	0	15	0	0	0	6	0	0	8	0	0	0	0	0	0	0	0	0	0	0	3	1	0	0	1010	0	0	0	0	2	0	0	0	2	0	0
SP325	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Kingella;oralis	4	0	0	0	15	0	0	0	2	2	0	0	24	5	6	0	0	0	6	5	0	9	17	4	0	6	0	335	17	32	19	4	10	7	0	5	5	3	0	8	0	0	14	0	9	21	7	10	3	0	2	2	2	3	0	15	0	61	0	4	23	34	26	0	5	0	31	6	9	22	27	18	0	3	0	0	5	0	5	3	0	3	0	0	0	6
SP326	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinobaculum;sp._oral_taxon_183	10	0	7	14	0	0	5	0	8	0	0	4	0	5	2	0	0	2	11	14	4	0	3	2	14	0	9	0	17	2	16	3	2	6	4	25	5	0	0	29	0	16	2	4	7	10	0	3	13	5	0	3	3	3	2	2	5	6	0	0	0	41	0	0	0	4	10	4	4	3	21	0	0	0	0	4	2	11	9	15	0	0	0	0	9	3
SP327	Bacteria;Actinobacteria;Coriobacteriia;Eggerthellales;Eggerthellaceae;Cryptobacterium;curtum	0	0	0	0	10	0	0	15	0	287	0	2	0	0	0	0	3	2	52	0	0	0	2	0	5	7	0	15	0	0	0	28	0	0	0	0	5	0	3	29	0	0	0	0	0	0	4	0	0	0	0	0	2	0	0	0	0	17	2	0	0	12	3	12	4	0	10	0	0	3	13	3	19	0	0	0	0	0	3	0	0	0	0	0	0	0
SP33	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;nucleatum_subsp._vincentii	0	87	3	3	440	4	5	6	101	0	6	0	6	10	6	0	26	28	565	115	4	29	0	2	0	7	0	28	7	161	13	19	0	5	1	14	6	13	0	225	5	27	0	11	34	68	45	24	148	15	12	38	3	17	4	16	23	936	16	8	0	35	3	3	42	0	542	21	4	6	28	3	12	31	19	11	0	10	4	218	2	2	2	31	7	0
SP331	Bacteria;Gracilibacteria_(GN02);GN02_[C-1];GN02_[O-1];GN02_[F-1];GN02_[G-1];sp._oral_taxon_872	0	200	0	0	0	0	2	0	7	0	0	0	0	0	3	0	0	7	3	0	0	0	0	0	0	5	0	0	7	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	4	0	0	7	0	3	0	0	0	0	8	2	0	21	0	0	0	0	0	0	0	2	0	0	6	0	0	0	0	50	0	0	6	0	9	6	0	0	2	0	0
SP34	Bacteria;Saccharibacteria_(TM7);TM7_[C-1];TM7_[O-1];TM7_[F-1];TM7_[G-6];sp._oral_taxon_870	55	163	0	18	13	17	444	13	135	47	21	5	41	0	112	37	42	13	83	43	16	21	115	13	4	49	5	3	11	2	7	8	4	2	1	5	33	10	144	74	49	16	2	23	14	43	3	50	32	81	32	0	18	12	22	138	36	7	0	4	11	8	0	34	11	8	4	9	0	10	7	0	45	13	84	12	42	98	0	0	1	6	2	43	6	2
SP35	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;parvula_group	255	620	5665	701	885	463	271	156	2168	461	1560	2260	3209	2744	1154	217	2203	2073	910	1307	2256	70	1816	7647	5854	948	155	1019	1782	2950	374	635	3368	2538	1659	1059	2166	871	1076	942	3036	295	210	1090	3229	1691	3881	672	1897	1889	1925	1009	2680	1925	2375	1120	2023	3405	1818	2698	1617	5310	907	315	1278	184	1977	1941	2037	2182	2665	1000	238	1011	1126	264	1336	1405	13	2488	258	3363	1930	375	176	249
SP36	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;oris	5	418	0	12	79	19	9	17	19	12	3	14	10	0	10	0	8	1	87	19	0	6	0	2	0	16	1	207	3	0	11	2	0	5	0	5	12	5	9	211	0	0	0	24	17	29	0	6	12	18	10	0	0	10	14	3	26	434	0	6	3	53	2	16	1	0	213	8	8	3	42	10	30	1	0	1	0	10	7	48	5	31	2	16	3	0
SP37	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Micrococcaceae;Rothia;mucilaginosa	5693	1237	5175	8011	4850	13854	2595	1315	3409	3673	8748	4251	3615	6873	3212	13644	4166	7027	1811	14789	6329	6617	4692	3151	5089	1558	2546	8123	9170	4936	5301	1518	6161	5691	746	1856	10959	1428	13594	17694	1716	318	388	1605	2109	2407	4157	5348	4031	4878	3833	9579	3240	5521	4865	14659	2236	3152	1111	3426	4443	3906	2217	848	19996	7038	3235	4782	3295	2992	5012	2388	1252	12845	652	10572	3276	1354	3651	2850	5702	583	5054	2035	2216	2496
SP38	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Dialister;invisus	0	33	0	4	45	0	0	8	19	33	18	3	31	36	74	6	35	46	60	27	2	3	8	9	21	12	3	93	27	2	22	16	5	10	8	6	4	14	7	122	4	0	0	13	14	14	14	6	13	5	10	14	4	5	11	13	87	102	120	9	5	110	7	2	7	0	58	8	6	5	111	7	6	7	18	2	6	32	7	14	4	3	14	16	2	0
SP39	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;odontolyticus	1188	236	862	108	511	646	661	68	9290	452	1053	682	152	1026	384	1178	273	530	256	708	751	255	550	728	260	421	344	916	1079	774	498	400	184	688	229	583	740	45	717	1726	1705	292	103	340	82	63	324	202	277	1745	885	41	632	613	217	180	435	143	96	1592	143	565	531	540	595	507	651	1074	188	327	440	531	451	420	364	677	758	52	547	815	183	1511	344	2168	201	250
SP4	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_172	1475	7	314	7	15	7	93	1080	237	1082	6	313	4	6	92	68	1552	8	936	1251	176	3	522	5	7	627	14	4	50	6	2374	321	147	13	4	218	413	152	0	40	405	116	156	241	168	216	613	12	60	36	161	9	299	81	4	295	763	78	79	171	84	1184	60	449	83	23	298	472	12	127	832	73	365	65	222	24	2154	3912	1771	2388	8	8	510	593	170	372
SP40	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_175	7	3	11	5	108	37	1	22	16	35	8	0	81	0	36	45	13	31	16	46	4	6	0	5	34	37	0	0	79	0	263	21	0	19	49	3	7	0	0	33	0	8	15	7	4	16	8	0	17	2	68	24	2	7	0	89	0	89	5	107	37	111	57	50	43	3	92	59	44	24	114	66	33	30	0	36	26	18	36	49	20	9	19	30	8	10
SP41	Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;morbillorum	14	84	7	1	60	39	41	56	16	67	238	25	111	29	12	11	32	58	17	66	20	212	6	44	15	30	50	97	356	85	15	5	4	32	31	174	67	30	301	16	28	14	0	67	3	56	7	32	64	50	14	15	13	48	59	73	84	57	92	19	54	10	19	21	17	9	44	68	11	113	12	59	10	12	35	15	24	717	25	9	393	144	34	32	15	19
SP42	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;tigurinus	44	72	0	8	244	286	1	266	111	122	33	89	493	176	68	11	761	42	84	59	22	43	10	9	245	347	16	242	13	173	192	115	8	83	6	400	462	25	88	32	2	38	12	24	13	0	38	4	68	67	13	36	37	21	132	112	41	288	69	17	50	39	205	15	58	3	115	229	237	77	41	225	18	51	34	39	7	65	84	37	386	69	0	33	0	24
SP44	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;denticola	0	116	0	0	23	3	0	0	1	7	0	30	0	4	16	11	110	0	111	13	0	15	0	0	80	0	0	0	5	24	30	55	0	0	0	0	5	0	0	171	0	0	0	0	11	60	5	2	6	5	19	4	2	0	31	13	8	59	17	0	10	359	6	0	4	4	28	0	13	6	225	1	0	2	0	2	0	4	0	2	0	0	0	0	0	0
SP45	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;sp._oral_taxon_149	4	0	0	2	34	0	10	116	139	3	0	66	16	0	17	0	4	49	17	132	49	8	0	0	36	2	12	0	10	0	103	98	39	650	91	0	4	2	10	10	22	0	0	0	259	8	103	95	12	28	28	64	29	3	11	11	76	2	349	236	268	259	0	42	0	3	3	86	17	70	154	15	28	0	123	4	61	389	124	181	0	1	10	39	6	11
SP46	Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;gingivalis	23	8	112	19	0	14	15	0	4	7	219	0	51	28	0	0	0	13	7	209	4	2	27	0	17	0	2	206	5	22	3	7	0	2	0	2	2	0	48	72	37	0	0	55	4	14	3	26	51	0	2	6	0	5	14	0	33	31	0	34	6	2	0	0	9	15	21	20	5	6	0	0	0	8	2	23	3	2	5	8	9	44	17	7	8	10
SP47	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_221	27	0	0	1	0	0	31	2	535	86	0	39	12	0	20	2	5	5	83	29	291	18	61	2	0	7	51	0	3	0	21	21	2	73	0	9	34	0	62	32	0	0	0	52	35	196	0	4	4	73	33	31	7	2	12	0	97	13	11	45	24	129	22	6	10	5	7	33	0	32	111	16	10	11	0	6	0	35	16	219	0	0	80	44	47	5
SP48	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcus;stomatis	110	285	597	8	35	39	103	27	82	18	412	5	53	19	27	0	55	336	44	138	17	46	195	106	46	20	190	139	101	505	4	8	9	70	13	42	6	127	6	334	181	43	18	201	32	10	7	19	113	51	77	46	14	53	228	34	89	32	148	105	9	20	30	23	112	72	40	94	23	8	21	27	30	92	262	101	24	37	65	24	93	222	91	211	74	93
SP5	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;parasanguinis_II	1147	14	231	646	309	153	3682	1170	702	1141	12	266	243	115	144	2654	758	209	230	8	1342	118	576	6	296	408	5	0	174	348	797	228	1135	2425	468	179	1422	265	315	300	158	195	89	149	231	202	589	285	95	994	632	167	3928	11	8	346	137	253	75	711	456	436	644	274	1398	75	380	463	1122	775	334	756	238	1036	18	179	680	224	1310	370	13	3	188	849	299	726
SP50	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_064	0	310	757	3677	2078	1180	67	301	890	8	0	671	885	0	1548	0	0	1000	308	0	0	0	613	308	11	0	80	23	0	0	5330	95	1570	30	12	142	0	1	192	0	16	31	238	222	0	0	275	187	0	0	419	57	46	13	0	155	95	582	81	9	118	12	14	293	0	789	638	102	40	0	704	25	84	1	1	873	0	1	943	4	7603	12	0	1821	1530	119
SP51	Bacteria;Actinobacteria;Coriobacteriia;Coriobacteriales;Coriobacteriaceae;Atopobium;parvulum	483	34	187	129	49	13	318	802	381	963	116	190	203	69	313	189	286	183	481	341	47	100	235	52	322	119	109	60	193	104	383	2175	303	344	252	237	145	76	197	241	1213	43	47	238	212	49	1024	51	82	85	296	27	583	45	56	39	106	15	184	99	185	244	213	666	127	75	204	96	140	167	312	242	714	104	150	151	332	431	538	823	14	105	243	2536	187	467
SP52	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;salivarius	555	66	22	2298	125	0	15364	5942	3282	11035	70	527	139	41	3015	4031	627	297	1880	163	159	17	51	0	66	2472	484	0	119	124	2154	3321	14012	3042	16163	0	47	36	2367	11	1048	133	133	196	2616	482	9488	243	3321	0	1578	0	6455	579	0	0	1960	10	212	0	2911	0	4	3687	1370	0	152	503	4351	2315	0	82	3316	880	2384	48	14633	11549	26595	33984	59	111	268	8192	968	4124
SP53	Bacteria;Firmicutes;Bacilli;Lactobacillales;Carnobacteriaceae;Granulicatella;elegans	1	260	95	872	158	18	173	208	12	44	5	11	215	1802	614	92	599	26	235	12	19	18	434	27	15	208	82	0	538	0	16	5	74	5	68	242	88	59	598	4	140	8	18	28	3	97	29	633	93	211	41	5	1	23	542	380	26	111	27	8	101	17	42	1054	100	58	58	40	3	735	37	50	889	66	4	85	268	217	77	3	2206	107	6	28	128	51
SP54	Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;sanguinis	1150	112	1435	364	94	388	499	31	92	41	1330	157	458	229	88	222	326	1269	60	819	222	87	368	745	287	353	477	401	2862	1225	135	12	218	605	81	1318	411	122	1251	200	473	71	63	360	84	68	52	96	149	151	293	43	862	442	187	370	150	397	82	213	65	433	112	20	608	246	257	305	95	131	368	114	34	483	144	341	370	327	144	127	454	619	118	114	178	145
SP55	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;endodontalis	0	1367	0	0	2	24	0	0	0	240	13	0	57	18	89	0	53	229	1844	0	0	75	0	0	2	328	2	160	329	20	0	0	0	4	3	0	17	98	0	600	30	0	0	12	117	99	70	34	497	1	12	47	4	38	550	46	8	292	127	3	5	0	3	46	84	14	160	4	4	6	2	0	24	68	0	20	2	93	0	1	424	2	1	38	51	0
SP56	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Filifactor;alocis	0	119	0	0	0	6	4	8	49	29	91	0	5	15	0	0	12	148	358	2	0	0	0	1	0	43	4	0	50	0	0	5	0	0	0	13	0	13	3	705	9	0	4	16	0	17	2	2	84	11	15	42	0	4	18	0	0	0	11	4	0	0	2	4	53	3	3	2	0	4	0	0	11	52	24	10	0	22	0	17	41	32	0	29	13	2
SP58	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Lachnoanaerobaculum;orale	93	109	0	7	0	0	13	38	143	21	147	27	14	2	3	0	16	8	22	32	24	6	12	48	43	14	0	5	0	0	6	70	36	10	16	2	21	8	1	34	52	12	0	41	2	12	64	0	5	15	19	3	11	3	44	0	104	0	17	12	17	0	7	0	2	12	0	26	11	18	4	16	0	0	0	19	79	79	26	76	5	50	0	34	33	16
SP59	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;vestibularis	0	0	0	0	1060	433	0	7509	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	21	0	0	0	0	0	0	0	0	1688	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SP6	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;australis	585	105	2164	169	1	353	73	62	86	533	2164	5	770	1908	306	276	0	1176	89	530	24	310	327	1467	0	169	482	882	534	1211	30	0	37	0	43	447	0	10	0	725	471	33	48	171	153	117	41	107	337	70	35	203	25	1166	156	29	841	329	105	273	68	372	0	121	0	175	200	136	361	82	268	0	101	0	252	249	371	185	20	6	1359	1213	130	202	36	183
SP60	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_061	75	37	0	760	5	1576	642	170	857	346	318	0	372	159	36	6	856	19	28	1792	190	63	1830	103	114	21	208	57	0	816	1211	196	93	587	163	816	240	44	257	0	650	124	80	163	72	104	0	230	224	1671	1852	187	977	38	88	66	78	60	162	357	496	45	445	93	0	388	215	146	364	266	437	468	264	0	5	729	368	222	718	58	0	66	271	904	113	292
SP61	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;nigrescens	5	6	2	0	0	6	0	6	0	4	0	10	3	11	6	3	1	15	38	3	0	52	0	3	0	0	0	15	2	19	15	7	5	5	3	0	9	3	18	10	0	0	0	3	9	42	40	0	28	6	12	19	2	0	66	12	61	154	64	7	0	110	0	5	0	0	72	7	9	0	67	0	2	0	2	0	0	195	7	2	5	4	0	0	2	0
SP62	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;intermedia	6	3	0	0	0	0	0	12	0	6	0	2	0	0	0	0	0	0	40	2	0	179	0	0	0	0	0	91	0	0	0	0	0	0	0	6	0	0	0	0	0	4	0	3	0	0	0	0	110	0	36	243	0	0	188	98	0	0	0	2	0	0	0	0	0	0	11	2	0	0	0	0	0	0	9	0	0	0	0	0	46	19	0	0	0	47
SP63	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Kingella;denitrificans	0	14	0	0	0	0	0	0	0	3	0	0	16	6	0	34	0	8	14	0	0	4	4	3	0	0	0	0	7	0	0	0	0	0	0	5	14	2	0	35	0	0	0	0	0	132	0	152	11	3	2	0	0	0	8	22	0	31	8	0	34	0	1	0	7	0	20	0	0	39	0	0	0	6	23	2	0	14	0	0	82	0	0	0	0	0
SP64	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp._oral_taxon_473	0	26	5	0	26	122	6	1	2	0	4	2	179	109	9	3	0	3	182	52	0	12	0	240	0	15	24	18	6	0	3	0	0	0	68	0	0	78	0	3	0	22	19	31	0	5	0	101	7	179	0	3	7	189	374	540	15	57	57	0	191	0	0	89	5	0	41	2	0	250	3	13	89	10	57	0	34	2	0	0	22	39	25	0	0	0
SP65	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;constellatus	3	29	0	0	13	0	0	0	3	4	12	2	0	0	29	15	20	49	34	7	0	5	13	0	0	7	2	56	67	272	57	7	4	28	0	4	0	0	0	23	17	7	26	0	30	4	0	5	83	16	0	3	0	6	14	0	1	35	9	9	0	6	24	0	55	5	28	17	8	9	6	20	1	30	3	9	5	15	0	0	5	3	0	41	14	13
SP66	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinobaculum;sp._oral_taxon_848	0	0	0	14	5	2	8	0	7	35	4	0	0	0	16	0	0	20	15	14	0	2	0	2	0	8	12	20	0	0	12	0	0	0	4	25	13	0	2	68	0	0	0	0	0	0	2	6	4	0	10	0	4	21	4	2	11	9	7	0	3	17	15	6	10	2	8	3	0	4	17	19	7	2	0	0	7	17	38	53	11	0	0	35	2	0
SP67	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Stomatobaculum;sp._oral_taxon_097	166	7	204	8	0	10	93	0	102	0	143	0	37	43	4	1	12	118	0	25	3	8	11	128	43	9	36	28	111	31	10	0	0	132	10	14	0	2	6	236	11	20	0	59	9	10	13	2	2	8	60	2	42	22	17	2	35	13	11	12	6	0	5	0	2	35	35	4	17	2	0	5	3	0	83	54	4	10	2	62	7	158	49	13	24	3
SP68	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;parainfluenzae	425	642	1827	236	445	309	19	45	203	13	1022	293	270	255	79	87	54	104	114	359	499	159	1066	2311	199	170	33	1734	591	1872	11	4	181	132	66	189	367	22	4470	89	275	54	67	112	618	88	78	1444	2231	737	420	430	19	745	1145	1369	395	1471	457	837	209	293	77	42	141	171	833	1301	439	214	210	83	35	123	711	163	344	279	109	303	1518	232	66	65	82	61
SP69	Bacteria;Actinobacteria;Actinobacteria;Corynebacteriales;Corynebacteriaceae;Corynebacterium;matruchotii	18	0	0	0	11	28	0	16	65	7	7	0	10	13	0	0	81	178	63	15	0	24	0	0	5	152	0	45	22	0	16	27	2	1	0	5	108	3	0	22	2	0	6	5	45	99	3	0	13	0	3	0	0	0	4	4	0	102	46	15	0	61	31	10	2	4	48	18	6	10	44	29	11	2	0	5	10	0	11	0	5	2	0	0	2	2
SP7	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;mitis	360	2038	3922	3844	5031	4639	2435	4810	575	1153	333	953	2613	3799	2777	4876	4924	0	1959	0	406	9860	1439	1564	528	12835	1928	3	4607	33	3628	288	3381	9	538	11545	1953	376	3877	5564	1645	92	103	299	39	4915	1522	11200	929	1198	941	2260	178	1305	1740	1856	733	3464	603	317	688	714	663	10061	5887	2389	1674	267	153	5072	3223	787	7446	3308	62	3250	1632	396	2620	295	7548	521	36	232	1030	5036
SP70	Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Solobacterium;moorei	290	226	436	160	28	26	291	107	86	249	95	162	232	143	170	8	210	1236	142	528	53	127	720	179	61	148	203	302	297	1967	94	228	24	129	77	266	108	81	93	519	1075	82	62	210	53	113	156	34	276	236	287	33	144	77	177	86	94	51	566	89	127	56	311	23	215	31	308	116	25	162	46	316	82	173	166	41	694	165	722	63	72	115	284	1175	204	158
SP71	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;salivae	303	37	13	27	52	2	70	368	105	320	7	385	67	6	40	60	63	18	95	90	192	53	88	26	32	60	85	50	2	0	105	244	250	293	146	159	58	41	276	43	59	63	43	50	529	291	497	26	167	208	211	52	620	68	47	82	364	42	1955	111	202	253	128	118	87	69	43	116	150	220	199	141	148	57	522	82	976	368	72	1439	4	42	63	170	50	99
SP72	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;parasanguinis_I	82	10	12	75	116	48	665	112	240	1069	56	236	257	12	200	1334	160	235	17	270	229	87	210	10	214	0	418	728	212	244	13	120	108	233	60	138	0	73	31	508	307	11	42	55	314	115	747	23	31	201	180	447	1507	97	16	3	149	23	102	162	117	166	53	674	154	199	23	366	910	445	122	187	493	88	66	306	318	264	161	631	0	72	92	407	107	143
SP74	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_225	0	1	5	50	13	15	6	5	15	0	0	0	78	19	5	0	16	14	12	9	4	9	0	12	0	7	4	0	27	27	3	5	0	0	29	2	3	1	7	2	4	0	5	8	0	51	0	46	6	7	2	0	0	0	0	4	9	50	27	0	5	0	2	2	0	6	23	0	6	10	3	2	2	2	0	7	20	4	0	52	0	4	0	4	0	2
SP75	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;cristatus	814	66	276	39	716	1038	475	352	8	191	405	1	1089	1602	206	409	1364	203	1002	111	47	169	453	1	393	603	22	71	763	432	703	108	103	53	50	395	144	124	117	189	241	30	11	9	489	98	160	172	21	55	23	84	44	16	777	507	50	143	166	409	34	42	185	55	206	330	243	289	114	900	1141	220	272	198	13	493	291	201	207	385	1413	55	44	26	60	69
SP76	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sanguinis	102	285	325	66	646	388	182	278	268	1118	125	8	364	117	198	101	11	811	101	517	38	116	198	125	8	191	18	180	338	75	56	95	38	74	39	79	393	23	78	242	43	7	0	54	260	61	87	141	320	46	64	34	60	141	14	80	48	1633	150	88	14	337	141	105	845	16	877	138	409	201	239	147	139	663	22	23	41	216	255	274	121	23	110	115	14	234
SP77	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp._oral_taxon_512	0	40	0	0	12	0	0	4	1	0	0	0	0	0	2	0	0	0	0	0	0	75	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	71	0	0	0	0	0	122	0	0	0	0	0	0	0	0	0	0	0	0	0	16	0	9	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0
SP78	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_314	0	218	0	0	0	0	0	4	0	7	2	52	0	82	505	0	0	0	21	38	0	101	496	2	0	0	562	66	0	108	0	0	38	733	0	0	0	5	0	0	0	8	8	0	410	264	0	63	0	6	0	0	109	97	453	163	0	0	128	0	1004	129	0	0	0	58	0	0	88	812	91	0	0	61	0	0	0	177	4	0	50	0	466	0	0	147
SP79	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;gerencseriae	0	4	10	0	0	2	4	0	8	2	0	0	0	0	0	0	10	17	9	6	2	0	29	8	8	0	7	1	0	0	0	0	0	0	0	2	4	2	2	72	0	2	0	0	11	0	2	7	9	4	3	6	2	3	5	36	2	20	0	0	4	70	0	0	23	0	13	4	2	2	56	6	0	16	0	0	0	45	14	67	2	2	0	6	2	0
SP8	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_058	592	841	305	1449	1	24	58	154	29	240	736	9	286	790	184	110	402	1653	34	1001	236	707	0	63	58	1797	40	489	863	15	146	18	15	156	212	584	433	81	520	274	0	36	38	79	39	1201	2	0	38	32	92	150	18	361	464	533	106	1040	67	131	65	22	216	15	50	298	572	99	0	0	201	211	30	27	1	478	157	373	5	4	1735	338	16	124	141	20
SP80	Bacteria;Firmicutes;Clostridia;Clostridiales;Ruminococcaceae;Ruminococcaceae_[G-2];sp._oral_taxon_085	35	562	94	30	300	5	17	12	170	76	49	15	7	10	157	4	41	85	30	43	8	11	31	10	0	77	13	20	40	96	16	19	9	84	33	29	0	13	23	30	169	0	10	9	128	23	195	49	12	27	43	19	44	18	28	5	72	0	305	0	30	34	42	14	6	12	87	7	22	25	19	30	11	9	393	26	0	38	38	77	20	46	2	19	2	6
SP82	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Lachnospiraceae_[G-2];sp._oral_taxon_096	661	8	0	3	4	3	81	77	380	140	13	4	28	3	612	5	10	353	143	60	70	23	70	44	5	75	258	0	0	2	165	48	2	52	238	22	3	20	0	66	230	35	9	63	430	150	3	3	42	240	168	90	11	49	34	20	526	6	96	238	403	112	2	205	0	35	8	135	5	339	81	0	182	0	4	51	169	289	204	77	5	217	42	223	98	10
SP83	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;noxia	6	346	0	2	1165	10	3	5	92	3	0	7	0	0	8	3	13	11	80	28	13	0	2	0	11	6	2	8	0	11	17	20	2	3	0	10	3	0	2	16	9	0	0	0	9	2	23	450	13	0	2	4	0	2	0	56	0	37	10	7	13	73	6	0	2	0	19	2	3	7	62	6	2	0	298	0	0	28	0	46	0	0	2	0	6	0
SP85	Bacteria;Firmicutes;Clostridia;Clostridiales;Lachnospiraceae_[XIV];Stomatobaculum;longum	54	0	0	0	12	1	0	89	70	19	0	25	0	0	4	11	40	0	38	7	4	15	6	0	3	1	0	0	0	0	22	80	50	107	0	0	16	13	15	0	96	0	0	1	6	21	36	0	5	10	0	1	1	1	0	3	8	0	11	3	13	35	9	4	3	1	0	0	2	14	38	9	8	3	0	2	13	11	18	43	0	0	0	12	0	21
SP87	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;sputorum	0	19	22	5	11	14	6	0	17	4	2	4	37	11	8	2	0	3	50	11	4	3	27	54	0	29	0	111	6	0	0	0	7	3	5	0	8	0	0	3	8	6	0	6	8	0	9	134	29	43	3	25	0	8	2	2	12	404	20	3	2	0	0	0	0	3	202	21	95	4	6	0	0	0	76	8	0	24	17	239	15	3	0	4	13	4
SP88	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;sp._oral_taxon_780	0	34	0	28	52	0	2	25	0	0	2	0	268	64	2	10	81	7	98	5	4	2	2	0	0	127	10	0	30	0	2	0	0	0	6	2	0	7	0	0	0	23	0	0	6	3	6	196	4	3	6	6	0	0	29	165	14	67	2	0	56	0	15	137	3	0	42	0	2	75	0	17	110	6	0	0	0	75	17	0	14	6	0	0	0	0
SP89	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;tannerae	6	25	10	4	6	5	41	7	0	4	119	7	255	44	128	2	0	224	116	20	0	58	3	14	40	36	195	36	6	18	1	0	0	2	195	73	0	98	0	5	2	10	0	52	52	2	0	0	36	45	77	589	31	182	1504	37	186	561	154	8	44	1	5	20	0	3	292	17	3	42	17	2	21	0	8	0	129	1	11	0	2	54	13	3	2	1
SP9	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_066	993	69	354	0	189	79	552	186	84	0	2757	508	0	71	44	13	114	1012	22	64	165	89	577	2344	0	279	153	170	1612	321	1	2	165	360	4	0	71	0	108	939	434	24	26	47	100	18	74	288	375	0	120	101	36	280	12	68	39	267	6	940	37	1	25	1	285	154	383	667	0	468	228	22	0	198	1	131	149	67	297	1073	257	325	21	42	26	101
SP90	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_392	0	14	0	0	18	3	7	3	69	0	100	0	7	4	4	0	4	25	26	31	3	3	10	28	0	5	2	0	18	9	0	0	0	0	0	1	9	0	16	1	2	0	0	0	0	10	30	5	5	0	0	2	3	0	8	8	8	0	5	0	2	0	0	2	2	0	0	4	0	11	0	0	2	0	41	2	3	25	4	0	15	0	0	9	0	0
SP91	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;melaninogenica	3998	2033	5750	826	223	640	11446	123	1267	851	6335	1301	2292	2043	3883	99	1717	3068	565	888	632	1753	5683	6797	1826	2573	14725	1048	156	2297	835	347	2001	8426	2378	4733	5974	2597	1923	2385	1662	262	263	2541	4138	1034	2036	1068	1689	4605	1941	2930	7034	3150	8087	3137	5714	335	8554	9768	2618	5554	1197	546	7184	2233	7494	5825	6115	3902	3766	1270	1747	4610	1940	2806	4624	1550	2688	7557	545	4016	10269	601	10586	1004
SP92	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_306	111	40	2	82	61	0	60	506	665	619	4	69	5	2	206	158	109	7	925	39	228	45	778	27	5	185	0	3	18	10	514	2844	5389	277	241	327	41	9	215	48	53	15	42	184	485	383	536	63	697	852	12	116	274	1	14	323	103	6	118	3	539	180	3	937	2	21	24	16	86	476	154	5	874	4	66	23	1459	475	663	9	7	4	239	2	0	798
SP94	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._oral_taxon_930	0	0	0	2	0	0	0	0	0	2	0	0	62	208	136	0	0	0	2	0	0	0	0	0	0	16	0	0	0	0	2	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	2	153	431	0	0	0	0	0	0	0	31	14	0	0	0	0	49	4	0	35	15	0	0	0	7	0	0	211	0	0	0	0	0
SP95	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;pallens	1698	31	64	119	301	35	213	128	968	168	237	498	276	165	467	6	68	35	343	161	273	294	219	75	406	25	53	9	5	1079	57	662	255	1221	407	194	62	393	341	38	357	163	30	213	942	476	757	39	396	876	1550	350	491	77	377	347	1666	160	2574	2547	542	1886	880	33	32	304	111	1971	191	594	1412	963	99	25	2411	408	673	570	356	244	7	2	234	358	290	34
SP96	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_313	566	0	3	236	10	8	1	119	244	113	0	0	66	12	51	72	178	382	195	90	107	278	429	4	1	349	781	5	400	247	1211	1775	188	499	2254	3	10	92	181	32	933	73	46	293	2047	1320	1146	4	602	88	1951	1308	328	1	2	87	2224	16	3	487	468	0	140	145	242	209	263	292	328	371	44	155	269	204	9	196	1649	102	271	0	5	12	0	5820	150	337
SP98	Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;leadbetteri	4	78	33	12	33	105	9	5	11	0	497	0	42	35	8	0	0	15	0	222	21	11	1	2	0	0	46	7	3	90	13	25	0	1	20	3	0	0	39	0	33	0	4	56	0	6	22	154	8	2	1	13	0	19	0	13	38	78	3	0	8	0	5	0	4	0	79	4	29	8	0	4	0	0	1909	5	0	5	47	17	0	165	13	0	5	0
SPN1	Bacteria;Saccharibacteria_(TM7);TM7_[C-1];TM7_[O-1];TM7_[F-1];TM7_[G-3];sp._oral_taxon_351_nov_92.457%	0	0	0	5	0	3	4	0	2	0	23	0	6	0	0	0	8	11	0	12	3	10	0	2	0	0	2	6	19	0	0	0	0	0	0	35	0	0	0	72	97	0	0	4	7	0	0	0	0	0	0	0	2	0	2	0	0	0	52	3	0	0	0	0	0	3	0	0	0	0	0	0	0	0	342	10	0	0	0	0	0	5	0	0	7	0
SPN10	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;caviae_nov_84.834%	0	0	15	5	0	0	0	0	0	0	0	0	17	14	12	0	2	0	0	0	4	0	15	49	0	2	0	0	6	8	0	2	3	11	9	0	3	4	0	0	32	0	0	0	0	2	0	2	8	6	13	6	3	3	0	1	24	9	16	31	5	3	0	0	0	0	0	18	6	4	7	0	0	0	10	0	0	3	4	0	0	39	11	0	2	0
SPN11	Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;sp._oral_taxon_020_nov_90.787%	0	0	0	0	0	0	0	0	6	0	0	0	122	97	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	3	0	0	62	63	0	0	0	0	2	0	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	15	0	0	0	0	0	2	0	0	0
SPN12	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_175_nov_97.826%	0	0	0	0	5	0	6	0	0	9	0	0	0	0	2	0	2	0	0	4	0	0	3	2	20	0	0	28	0	58	0	0	0	0	0	0	0	2	0	41	0	0	14	9	0	11	0	0	0	0	0	0	0	0	0	8	0	0	4	2	0	0	0	0	0	0	0	9	11	0	49	0	0	2	5	4	0	0	7	48	0	0	0	0	0	2
SPN13	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XIII];Parvimonas;sp._Oral_Taxon_A91_nov_97.345%	9	176	229	12	6	6	5	0	5	2	267	0	135	52	33	0	2	674	0	94	0	8	156	334	0	3	73	436	47	989	3	0	2	18	16	51	13	20	18	161	164	0	33	82	0	0	0	0	61	0	41	9	16	17	79	10	121	2	142	5	0	30	3	0	2	2	185	41	3	0	31	2	0	3	71	2	19	0	2	0	57	146	75	113	17	0
SPN14	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_175_nov_97.835%	0	0	0	0	0	0	5	0	6	0	0	0	0	0	0	0	4	0	0	0	2	0	0	0	0	0	0	0	0	0	102	0	0	1	0	0	0	0	0	6	0	0	15	2	0	34	0	0	0	13	11	0	0	0	0	18	11	0	0	0	23	0	0	0	37	0	0	0	0	16	0	1	0	34	0	0	0	0	0	0	2	0	0	11	7	0
SPN15	Bacteria;Firmicutes;Clostridia;Clostridiales;Clostridiales_[F];Clostridiales_[G];sp._Oral_Taxon_A96_nov_90.733%	0	34	0	0	0	18	0	0	0	0	0	0	0	0	0	0	0	0	0	39	0	0	0	10	0	0	0	0	43	0	0	0	0	0	0	0	0	0	0	188	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	19	0	0	0	0	0	0	0	9	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SPN16	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;actinomycetemcomitans_nov_97.826%	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	254	0	0	0	0	0	0	0	0	102	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SPN2	Bacteria;Firmicutes;Clostridia;Clostridiales;Peptostreptococcaceae_[XI];Peptostreptococcaceae_[XI][G-5];sp._oral_taxon_493_nov_95.652%	0	437	0	0	0	2	8	0	0	0	19	0	0	7	4	0	0	51	0	117	0	0	0	12	0	0	4	0	24	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	7	0	0	0	2	0	6	0	2	0	41	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	0	0	2	0
SPN20	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Micrococcaceae;Rothia;mucilaginosa_nov_92.731%	20	2	35	22	1	4	2	0	11	0	106	4	41	60	0	0	5	87	0	121	3	20	13	0	46	2	3	62	40	16	0	0	8	21	0	0	2	9	23	62	8	0	0	0	6	10	0	1	21	17	38	36	2	24	33	80	51	12	7	44	5	17	5	0	7	31	13	22	8	4	5	2	0	4	1	32	0	0	6	1	21	0	36	3	6	0
SPN25	Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Bergeyella;sp._oral_taxon_931_nov_92.609%	0	0	4	15	0	0	0	5	21	0	3	2	1180	167	325	16	0	0	229	17	2	0	326	0	0	0	27	0	13	0	11	3	9	2	0	2	0	3	167	0	0	0	0	0	0	48	3	0	2	46	0	0	0	0	70	93	16	2	23	0	13	0	63	0	2	44	0	0	0	55	0	60	3	0	0	49	298	0	4	0	330	8	4	67	2	0
SPN3	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._oral_taxon_064_nov_97.609%	2	0	0	0	0	0	3	60	0	14	0	1	0	0	8	10	0	0	1	0	5	0	4	0	0	5	1	0	0	0	4	1	45	1	15	0	2	1	2	0	1	0	0	0	14	4	13	0	1	1	6	0	3	3	0	0	5	0	0	1	1	1	0	18	0	0	2	0	0	32	0	1	9	11	0	1	16	11	185	17	0	0	7	14	0	26
SPN31	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;melaninogenica_nov_97.987%	101	0	0	0	0	2	3	0	0	8	0	0	0	2	0	0	0	1	0	0	0	0	39	0	0	0	100	0	0	0	0	0	0	145	0	4	0	40	0	1	12	0	0	0	158	0	0	0	0	81	0	28	322	0	0	0	0	0	0	45	72	64	0	0	34	0	0	29	0	0	0	0	0	33	0	0	0	0	1	0	2	0	74	0	43	0
SPN37	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_215_nov_96.963%	0	20	337	29	0	0	39	2	0	0	96	0	0	4	35	0	0	32	0	0	0	0	2	147	0	0	118	147	139	0	0	0	0	0	239	0	2	0	0	5	42	0	0	32	0	0	0	0	0	0	0	2	0	38	9	0	0	0	16	0	0	0	0	0	0	16	40	0	0	0	0	0	0	0	437	20	0	0	0	0	9	285	0	11	8	0
SPN4	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Selenomonas;sputigena_nov_97.162%	5	0	0	0	0	0	25	0	39	0	6	0	0	0	3	0	0	5	0	0	0	2	0	0	0	0	0	0	2	0	0	3	0	31	0	0	0	2	0	8	7	14	0	0	30	3	0	0	0	2	0	5	0	1	5	0	0	0	24	34	91	0	0	0	0	0	0	26	0	86	0	0	0	0	0	0	3	96	0	0	0	8	2	0	3	0
SPN42	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp._oral_taxon_308_nov_97.192%	0	3	0	0	0	0	0	0	0	0	0	63	0	0	0	0	0	3	0	2	0	6	0	0	0	0	47	0	0	0	0	0	9	9	0	159	0	0	0	0	0	31	0	0	8	0	34	0	0	495	94	67	0	19	0	0	0	0	94	0	30	0	6	4	0	16	0	0	20	25	0	0	0	0	0	16	0	0	0	0	0	0	0	0	0	0
SPN49	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Mobiluncus;curtisii_nov_96.725%	0	58	0	0	0	3	0	126	5	95	20	2	7	3	6	0	3	5	2	240	0	21	0	2	0	2	0	0	0	2	8	10	2	11	0	23	3	11	0	55	161	0	0	12	10	15	765	16	0	0	5	0	37	8	8	0	0	2	9	7	0	0	0	0	0	0	18	15	0	0	0	0	2	0	124	0	10	7	0	0	0	11	29	90	2	0
SPN5	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Micrococcaceae;Rothia;mucilaginosa_nov_97.349%	0	0	0	3	0	0	0	0	0	0	0	0	34	0	0	194	6	0	0	0	0	4	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	3	13	0	0	0	0	0	43	0	0	51	103	6	0	5	0	0	0	0	0	0	14	0	0	0	0	0	0	0	0	0	7	0	0	0	0	0	0	0	0	0	0
SPN53	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;parasanguinis_II_nov_97.826%	0	0	0	0	0	304	0	0	0	0	0	42	0	0	0	0	6	70	0	0	494	12	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	15	25	0	0	0	0	0	7	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	14	0	0	0	0	95	0	0	0	0	0	93	0	0
SPN6	Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;haemolysans_nov_97.511%	0	0	2	0	2	3	0	0	2	3	0	6	7	11	0	0	7	2	2	0	1	18	0	0	0	41	7	0	17	0	0	0	1	0	2	2	2	0	15	7	0	0	0	0	0	0	4	10	1	4	0	0	4	20	5	0	0	35	0	0	2	0	0	0	0	8	12	3	1	10	0	0	3	0	0	0	7	0	0	0	190	0	0	0	0	0
SPN61	Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp._oral_taxon_215_nov_96.529%	6	10	2	0	5	7	2	16	76	0	1334	0	18	6	0	0	0	11	0	11	6	0	81	0	0	4	0	0	1	0	0	0	0	0	37	0	0	0	0	92	200	0	0	7	0	0	0	0	16	0	0	3	0	21	0	1	0	0	13	29	0	0	16	0	0	0	0	18	0	0	0	13	0	0	0	0	0	5	0	2	3	0	0	0	0	0
SPN64	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;histicola_nov_97.609%	0	0	0	0	0	5	114	0	0	0	0	486	0	8	0	0	0	0	0	0	0	0	140	0	0	0	20	0	0	1	0	0	0	0	23	9	0	80	0	0	0	0	0	24	0	0	0	0	0	0	0	0	0	0	14	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	102	0	0	0	0	0	0	152	0	0	0
SPN7	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp._oral_taxon_284_nov_97.614%	0	6	0	0	0	0	3	0	0	0	0	0	17	3	11	4	4	0	0	0	4	0	0	2	0	0	16	0	12	0	0	3	0	0	7	7	0	5	0	0	0	0	10	0	0	0	0	113	0	46	0	76	0	0	2	61	18	20	0	0	0	0	0	0	0	0	8	0	13	0	0	0	0	0	0	0	0	0	0	0	0	0	0	6	0	0
SPN73	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp._Oral_Taxon_E27_nov_85.783%	11	0	68	14	2	0	0	0	0	2	251	5	82	113	20	0	7	35	4	70	3	34	11	122	0	11	7	51	64	24	3	5	2	10	16	8	2	5	8	13	0	0	0	0	0	12	0	5	17	16	30	15	5	13	53	25	23	40	1	20	9	2	12	2	4	8	38	19	13	13	0	10	1	5	22	26	0	6	9	8	99	33	19	3	5	0
SPN75	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp._oral_taxon_473_nov_97.403%	0	0	0	0	0	2	0	0	0	0	0	0	67	0	0	0	0	0	0	0	0	2	0	5	0	4	0	0	4	0	0	0	4	2	0	0	0	0	0	0	0	0	0	13	9	0	0	0	75	129	1	21	0	10	142	151	0	0	91	0	12	0	0	0	0	1	0	0	0	13	0	0	0	0	160	2	1	2	0	0	0	53	2	0	0	0
SPN8	Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;sanguinis_nov_99.095%	0	3	0	2	0	7	30	7	4	0	16	8	18	15	3	13	4	7	0	0	0	0	5	0	0	12	2	0	10	8	5	0	0	2	3	0	16	11	0	8	3	2	0	3	2	4	0	6	9	12	9	4	0	1	8	21	15	3	0	0	8	12	6	2	8	0	3	0	10	10	13	0	3	5	5	3	15	5	9	0	0	0	0	6	1	8
SPN85	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;histicola_nov_96.957%	0	0	0	0	0	0	0	0	0	2	0	0	0	0	760	0	0	0	0	0	0	61	0	26	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	0	0	0	0	0	613	0	0	1	0	0	0	0	21	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
SPN86	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._oral_taxon_305_nov_93.723%	5	24	9	0	0	0	18	0	0	0	3	0	23	4	28	0	0	3	0	5	0	3	3	5	25	0	53	0	0	0	0	0	0	0	24	0	0	6	9	44	26	22	0	31	11	12	0	0	0	0	7	2	1	12	16	0	241	2	64	6	0	0	0	0	0	0	2	8	0	0	0	3	0	0	89	2	0	3	10	2	3	54	0	5	37	0
SPN9	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;sp._oral_taxon_180_nov_97.826%	98	2	0	2	0	0	2	131	62	1333	16	2	1278	5	35	0	41	0	23	0	0	24	269	0	2	4	2	0	3	6	23	1123	2	297	0	157	2	24	2	472	0	3	10	27	281	3	0	3	5	0	4	21	30	72	0	2	0	2	173	0	51	3	4	0	0	16	2	0	0	47	5	1	173	2	527	18	0	632	565	3	3	0	2	576	2	4
SPN95	Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;sp._Oral_Taxon_G21_nov_97.162%	0	0	0	0	0	0	0	2	2	0	0	0	0	0	5	0	0	0	6	0	0	4	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	30	0	0	0	0	2	2	2	0	465	0	0	0	0	0	0	0	0	220	10	0	0	2	0	3	0	0	0	0	0	0	0	2	0	0	0	2	0
SPP1	Bacteria;Actinobacteria;Actinobacteria;Actinomycetales;Actinomycetaceae;Actinomyces;multispecies_spp1_2	0	0	0	0	0	0	177	0	0	0	0	0	0	81	0	0	0	0	0	136	0	0	0	0	0	15	0	0	19	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	88	0	0	0	0	0	113	0	0	0	0	0	0	0	0	0	0	0
SPP11	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp11_2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	154	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	619	0	0	0
SPP12	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp12_2	1194	140	15	1849	5	633	0	0	3493	1029	21	555	901	67	4	443	853	1506	1397	234	5001	213	4532	454	38	628	1188	151	232	1532	24	0	41	980	0	236	3113	1262	943	1803	1277	78	91	1598	0	1418	0	875	1565	3029	2517	773	0	740	320	829	576	602	355	3301	582	4258	683	235	3717	300	857	2367	834	2546	2920	963	337	2464	0	1467	0	0	0	0	46	24	4625	2755	76	282
SPP13	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp13_2	0	0	0	0	0	0	0	0	0	0	0	0	378	0	0	0	0	0	0	0	0	0	148	125	0	0	611	54	239	0	0	0	0	0	0	0	0	0	0	0	0	0	8	0	0	0	0	0	97	203	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	59	0	0	0	0	0
SPP14	Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;multispecies_spp14_2	0	0	0	0	0	0	0	39	0	0	0	0	0	0	0	0	70	0	43	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	43	0	0	0	0	0	0	0	0	474	0	0	0	0	0	0	0	0	0	0	0	0	456	0	0	82	0	0	0	0	177	63	0	0	67	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SPP2	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp2_2	0	0	167	0	0	0	0	0	44	0	0	0	0	0	0	112	0	0	0	634	0	0	56	0	0	0	0	0	0	392	0	0	24	0	0	486	0	30	151	0	0	0	9	0	0	0	0	0	0	0	76	0	0	0	0	107	119	0	35	9	37	0	0	0	226	0	0	0	0	0	0	0	0	57	97	0	0	0	0	0	0	0	182	0	0	0
SPP4	Bacteria;Firmicutes;Negativicutes;Selenomonadales;Veillonellaceae;Veillonella;multispecies_spp4_2	50	0	0	612	0	0	0	0	616	0	0	0	0	0	67	0	13	0	35	0	0	24	0	0	0	0	1	0	0	0	0	206	0	450	0	0	0	0	0	0	0	0	0	0	0	15	0	0	0	153	0	212	0	0	0	0	211	1	0	0	184	0	63	0	0	5	0	46	787	0	0	164	0	0	0	12	567	0	28	0	0	0	0	16	0	0
SPP5	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp5_3	0	0	0	0	0	0	0	1351	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SPPN1	Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_sppn1_2_nov_97.835%	4	3	0	0	82	0	0	2	242	36	0	66	0	0	0	6	229	0	1	60	6	142	0	2	0	246	1	130	0	0	18	113	3	1034	0	213	3	30	290	110	1128	2	0	0	27	142	186	0	62	0	5	0	780	0	2	1	38	0	72	4	81	2	338	110	36	54	0	172	59	44	2	378	103	34	6	58	26	0	63	2	3	0	0	0	0	8
SPPN2	Bacteria;Actinobacteria;Actinobacteria;Bifidobacteriales;Bifidobacteriaceae;Alloscardovia;multispecies_sppn2_2_nov_95.022%	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	278	0	0	0	0	0	0	0	0	235	0	0	0	0	0	0	0	0	2	0	0	0	0

Download OTU Tables at Different Taxonomy Levels
Phylum	Count*:	Relative**:	CLR***:
Class	Count*:	Relative**:	CLR***:
Order	Count*:	Relative**:	CLR***:
Family	Count*:	Relative**:	CLR***:
Genus	Count*:	Relative**:	CLR***:
Species	Count*:	Relative**:	CLR***:
* Read count
** Relative abundance (count/total sample count)
*** Centered log ratio transformed abundance

;

The species listed in the table has full taxonomy and a dynamically assigned species ID specific to this report. When some reads match with the reference sequences of more than one species equally (i.e., same percent identiy and alignmnet coverage), they can't be assigned to a particular species. Instead, they are assigned to multiple species with the species notaton "s__multispecies_spp2_2". In this notation, spp2 is the dynamic ID assigned to these reads that hit multiple sequences and the "_2" at the end of the notation means there are two species in the spp2.

You can look up which species are included in the multi-species assignment, in this table below:

Another type of notation is "s__multispecies_sppn2_2", in which the "n" in the sppn2 means it's a potential novel species because all the reads in this species have < 98% idenity to any of the reference sequences. They were grouped together based on de novo OTU clustering at 98% identity cutoff. And then a representative sequence was chosed to BLASTN search against the reference database to find the closest match (but will still be < 98%). This representative sequence also matched equally to more than one species, hence the "spp" was given in the label.

Taxonomy Bar Plots for All Samples

Taxonomy Bar Plots for Individual Comparison Groups

Comparison No.	Comparison Name	Families		Genera		Species
Comparison 1	case vs control	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 2	Male vs Female	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 3	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 4	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 5	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 6	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 7	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 8	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 9	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 10	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 11	Desirable vs Low	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 12	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 13	Normal vs BorderlineHigh vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 14	BorderlineHigh vs Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 15	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 16	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 17	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG

VIII. Analysis - Alpha Diversity

In ecology, alpha diversity (α-diversity) is the mean species diversity in sites or habitats at a local scale. The term was introduced by R. H. Whittaker[1][2] together with the terms beta diversity (β-diversity) and gamma diversity (γ-diversity). Whittaker's idea was that the total species diversity in a landscape (gamma diversity) is determined by two different things, the mean species diversity in sites or habitats at a more local scale (alpha diversity) and the differentiation among those habitats (beta diversity).

References:
Whittaker, R. H. (1960) Vegetation of the Siskiyou Mountains, Oregon and California. Ecological Monographs, 30, 279–338. doi:10.2307/1943563
Whittaker, R. H. (1972). Evolution and Measurement of Species Diversity. Taxon, 21, 213-251. doi:10.2307/1218190

Alpha Diversity Analysis by Rarefaction

Diversity measures are affected by the sampling depth. Rarefaction is a technique to assess species richness from the results of sampling. Rarefaction allows the calculation of species richness for a given number of individual samples, based on the construction of so-called rarefaction curves. This curve is a plot of the number of species as a function of the number of samples. Rarefaction curves generally grow rapidly at first, as the most common species are found, but the curves plateau as only the rarest species remain to be sampled.

References:
Willis AD. Rarefaction, Alpha Diversity, and Statistics. Front Microbiol. 2019 Oct 23;10:2407. doi: 10.3389/fmicb.2019.02407. PMID: 31708888; PMCID: PMC6819366.

Boxplot of Alpha-diversity Indices

The two main factors taken into account when measuring diversity are richness and evenness. Richness is a measure of the number of different kinds of organisms present in a particular area. Evenness compares the similarity of the population size of each of the species present. There are many different ways to measure the richness and evenness. These measurements are called "estimators" or "indices". Below is a diversity of 3 commonly used indices showing the values for all the samples (dots) and in groups (boxes).

Alpha Diversity Box Plots for All Groups

Alpha Diversity Box Plots for Individual Comparisons

Comparison 1	case vs control	View in PDF	View in SVG
Comparison 2	Male vs Female	View in PDF	View in SVG
Comparison 3	no vs yes	View in PDF	View in SVG
Comparison 4	no vs yes	View in PDF	View in SVG
Comparison 5	no vs yes	View in PDF	View in SVG
Comparison 6	no vs yes	View in PDF	View in SVG
Comparison 7	no vs yes	View in PDF	View in SVG
Comparison 8	no vs yes	View in PDF	View in SVG
Comparison 9	Normal vs High	View in PDF	View in SVG
Comparison 10	Normal vs High	View in PDF	View in SVG
Comparison 11	Desirable vs Low	View in PDF	View in SVG
Comparison 12	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	View in PDF	View in SVG
Comparison 13	Normal vs BorderlineHigh vs High	View in PDF	View in SVG
Comparison 14	BorderlineHigh vs Normal vs High	View in PDF	View in SVG
Comparison 15	High vs Normal	View in PDF	View in SVG
Comparison 16	High vs Normal	View in PDF	View in SVG
Comparison 17	Normal vs High	View in PDF	View in SVG

Group Significance of Alpha-diversity Indices

To test whether the alpha diversity among different comparison groups are different statisticall, we use the Kruskal Wallis H test provided the "alpha-group-significance" fucntion in the QIIME 2 diversity package. Kruskal Wallis H test is the non parametric alternative to the One Way ANOVA. Non parametric means that the test doesn’t assume your data comes from a particular distribution. The H test is used when the assumptions for ANOVA aren’t met (like the assumption of normality). It is sometimes called the one-way ANOVA on ranks, as the ranks of the data values are used in the test rather than the actual data points. The test determines whether the medians of two or more groups are different.

Below are the Kruskal Wallis H test results for each comparison based on three different alpha diversity measures: 1) Observed species (features), 2) Shannon index, and 3) Simpson index.

Comparison 1.	case vs control	Observed Features	Shannon Index	Simpson Index
Comparison 2.	Male vs Female	Observed Features	Shannon Index	Simpson Index
Comparison 3.	no vs yes	Observed Features	Shannon Index	Simpson Index
Comparison 4.	no vs yes	Observed Features	Shannon Index	Simpson Index
Comparison 5.	no vs yes	Observed Features	Shannon Index	Simpson Index
Comparison 6.	no vs yes	Observed Features	Shannon Index	Simpson Index
Comparison 7.	no vs yes	Observed Features	Shannon Index	Simpson Index
Comparison 8.	no vs yes	Observed Features	Shannon Index	Simpson Index
Comparison 9.	Normal vs High	Observed Features	Shannon Index	Simpson Index
Comparison 10.	Normal vs High	Observed Features	Shannon Index	Simpson Index
Comparison 11.	Desirable vs Low	Observed Features	Shannon Index	Simpson Index
Comparison 12.	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	Observed Features	Shannon Index	Simpson Index
Comparison 13.	Normal vs BorderlineHigh vs High	Observed Features	Shannon Index	Simpson Index
Comparison 14.	BorderlineHigh vs Normal vs High	Observed Features	Shannon Index	Simpson Index
Comparison 15.	High vs Normal	Observed Features	Shannon Index	Simpson Index
Comparison 16.	High vs Normal	Observed Features	Shannon Index	Simpson Index
Comparison 17.	Normal vs High	Observed Features	Shannon Index	Simpson Index

IX. Analysis - Beta Diversity

NMDS and PCoA Plots

Beta diversity compares the similarity (or dissimilarity) of microbial profiles between different groups of samples. There are many different similarity/dissimilarity metrics. In general, they can be quantitative (using sequence abundance, e.g., Bray-Curtis or weighted UniFrac) or binary (considering only presence-absence of sequences, e.g., binary Jaccard or unweighted UniFrac). They can be even based on phylogeny (e.g., UniFrac metrics) or not (non-UniFrac metrics, such as Bray-Curtis, etc.).

For microbiome studies, species profiles of samples can be compared with the Bray-Curtis dissimilarity, which is based on the count data type. The pair-wise Bray-Curtis dissimilarity matrix of all samples can then be subject to either multi-dimensional scaling (MDS, also known as PCoA) or non-metric MDS (NMDS).

MDS/PCoA is a scaling or ordination method that starts with a matrix of similarities or dissimilarities between a set of samples and aims to produce a low-dimensional graphical plot of the data in such a way that distances between points in the plot are close to original dissimilarities.

NMDS is similar to MDS, however it does not use the dissimilarities data, instead it converts them into the ranks and use these ranks in the calculation.

In our beta diversity analysis, Bray-Curtis dissimilarity matrix was first calculated and then plotted by the PCoA and NMDS separately. Below are beta diveristy results for all groups together:

NMDS and PCoA Plots for All Groups

The above PCoA and NMDS plots are based on count data. The count data can also be transformed into centered log ratio (CLR) for each species. The CLR data is no longer count data and cannot be used in Bray-Curtis dissimilarity calculation. Instead CLR can be compared with Euclidean distances. When CLR data are compared by Euclidean distance, the distance is also called Aitchison distance.

Below are the NMDS and PCoA plots of the Aitchison distances of the samples:

NMDS and PCoA Plots for Individual Comparisons

Comparison No.	Comparison Name	NMDA				PCoA
Comparison No.	Comparison Name	Bray-Curtis		CLR Euclidean		Bray-Curtis		CLR Euclidean
Comparison 1	case vs control	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 2	Male vs Female	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 3	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 4	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 5	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 6	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 7	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 8	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 9	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 10	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 11	Desirable vs Low	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 12	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 13	Normal vs BorderlineHigh vs High	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 14	BorderlineHigh vs Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 15	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 16	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 17	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG	PDF	SVG

Interactive 3D PCoA Plots - Bray-Curtis Dissimilarity

Interactive 3D PCoA Plots - Euclidean Distance

Interactive 3D PCoA Plots - Correlation Coefficients

Group Significance of Beta-diversity Indices

To test whether the between-group dissimilarities are significantly greater than the within-group dissimilarities, the "beta-group-significance" function provided in the QIIME 2 "diversity" package was used with PERMANOVA (permutational multivariate analysis of variance) chosen s the group significan testing method.

Three beta diversity matrics were used: 1) Bray–Curtis dissimilarity 2) Correlation coefficient matrix , and 3) Aitchison distance (Euclidean distance between clr-transformed compositions).

Comparison 1.	case vs control	Bray–Curtis	Correlation	Aitchison
Comparison 2.	Male vs Female	Bray–Curtis	Correlation	Aitchison
Comparison 3.	no vs yes	Bray–Curtis	Correlation	Aitchison
Comparison 4.	no vs yes	Bray–Curtis	Correlation	Aitchison
Comparison 5.	no vs yes	Bray–Curtis	Correlation	Aitchison
Comparison 6.	no vs yes	Bray–Curtis	Correlation	Aitchison
Comparison 7.	no vs yes	Bray–Curtis	Correlation	Aitchison
Comparison 8.	no vs yes	Bray–Curtis	Correlation	Aitchison
Comparison 9.	Normal vs High	Bray–Curtis	Correlation	Aitchison
Comparison 10.	Normal vs High	Bray–Curtis	Correlation	Aitchison
Comparison 11.	Desirable vs Low	Bray–Curtis	Correlation	Aitchison
Comparison 12.	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	Bray–Curtis	Correlation	Aitchison
Comparison 13.	Normal vs BorderlineHigh vs High	Bray–Curtis	Correlation	Aitchison
Comparison 14.	BorderlineHigh vs Normal vs High	Bray–Curtis	Correlation	Aitchison
Comparison 15.	High vs Normal	Bray–Curtis	Correlation	Aitchison
Comparison 16.	High vs Normal	Bray–Curtis	Correlation	Aitchison
Comparison 17.	Normal vs High	Bray–Curtis	Correlation	Aitchison

X. Analysis - Differential Abundance

16S rRNA next generation sequencing (NGS) generates a fixed number of reads that reflect the proportion of different species in a sample, i.e., the relative abundance of species, instead of the absolute abundance. In Mathematics, measurements involving probabilities, proportions, percentages, and ppm can all be thought of as compositional data. This makes the microbiome read count data “compositional” (Gloor et al, 2017). In general, compositional data represent parts of a whole which only carry relative information (http://www.compositionaldata.com/).

The problem of microbiome data being compositional arises when comparing two groups of samples for identifying “differentially abundant” species. A species with the same absolute abundance between two conditions, its relative abundances in the two conditions (e.g., percent abundance) can become different if the relative abundance of other species change greatly. This problem can lead to incorrect conclusion in terms of differential abundance for microbial species in the samples.

When studying differential abundance (DA), the current better approach is to transform the read count data into log ratio data. The ratios are calculated between read counts of all species in a sample to a “reference” count (e.g., mean read count of the sample). The log ratio data allow the detection of DA species without being affected by percentage bias mentioned above

In this report, a compositional DA analysis tool “ANCOM” (analysis of composition of microbiomes) was used. ANCOM transforms the count data into log-ratios and thus is more suitable for comparing the composition of microbiomes in two or more populations. "ANCOM" generates a table of features with W-statistics and whether the null hypothesis is rejected. The “W” is the W-statistic, or number of features that a single feature is tested to be significantly different against. Hence the higher the "W" the more statistical sifgnificane that a feature/species is differentially abundant.

References:

Gloor GB, Macklaim JM, Pawlowsky-Glahn V, Egozcue JJ. Microbiome Datasets Are Compositional: And This Is Not Optional. Front Microbiol. 2017 Nov 15;8:2224. doi: 10.3389/fmicb.2017.02224. PMID: 29187837; PMCID: PMC5695134.

Mandal S, Van Treuren W, White RA, Eggesbø M, Knight R, Peddada SD. Analysis of composition of microbiomes: a novel method for studying microbial composition. Microb Ecol Health Dis. 2015 May 29;26:27663. doi: 10.3402/mehd.v26.27663. PMID: 26028277; PMCID: PMC4450248.

Lin H, Peddada SD. Analysis of compositions of microbiomes with bias correction. Nat Commun. 2020 Jul 14;11(1):3514. doi: 10.1038/s41467-020-17041-7. PMID: 32665548; PMCID: PMC7360769.

ANCOM Differential Abundance Analysis

ANCOM Results for Individual Comparisons

Comparison No.	Comparison Name
Comparison 1.	case vs control
Comparison 2.	Male vs Female
Comparison 3.	no vs yes
Comparison 4.	no vs yes
Comparison 5.	no vs yes
Comparison 6.	no vs yes
Comparison 7.	no vs yes
Comparison 8.	no vs yes
Comparison 9.	Normal vs High
Comparison 10.	Normal vs High
Comparison 11.	Desirable vs Low
Comparison 12.	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal
Comparison 13.	Normal vs BorderlineHigh vs High
Comparison 14.	BorderlineHigh vs Normal vs High
Comparison 15.	High vs Normal
Comparison 16.	High vs Normal
Comparison 17.	Normal vs High

ANCOM-BC Differential Abundance Analysis

Starting with version V1.2, we also include the results of ANCOM-BC (Analysis of Compositions of Microbiomes with Bias Correction) (Lin and Peddada 2020). ANCOM-BC is an updated version of "ANCOM" that: (a) provides statistically valid test with appropriate p-values, (b) provides confidence intervals for differential abundance of each taxon, (c) controls the False Discovery Rate (FDR), (d) maintains adequate power, and (e) is computationally simple to implement. The bias correction (BC) addresses a challenging problem of the bias introduced by differences in the sampling fractions across samples. This bias has been a major hurdle in performing DA analysis of microbiome data. ANCOM-BC estimates the unknown sampling fractions and corrects the bias induced by their differences among samples. The absolute abundance data are modeled using a linear regression framework.

References:

Lin H, Peddada SD. Analysis of compositions of microbiomes with bias correction. Nat Commun. 2020 Jul 14;11(1):3514. doi: 10.1038/s41467-020-17041-7. PMID: 32665548; PMCID: PMC7360769.

ANCOM-BC Results for Individual Comparisons

Comparison No.	Comparison Name
Comparison 1.	case vs control
Comparison 2.	Male vs Female
Comparison 3.	no vs yes
Comparison 4.	no vs yes
Comparison 5.	no vs yes
Comparison 6.	no vs yes
Comparison 7.	no vs yes
Comparison 8.	no vs yes
Comparison 9.	Normal vs High
Comparison 10.	Normal vs High
Comparison 11.	Desirable vs Low
Comparison 12.	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal
Comparison 13.	Normal vs BorderlineHigh vs High
Comparison 14.	BorderlineHigh vs Normal vs High
Comparison 15.	High vs Normal
Comparison 16.	High vs Normal
Comparison 17.	Normal vs High

LEfSe - Linear Discriminant Analysis Effect Size

LEfSe (Linear Discriminant Analysis Effect Size) is an alternative method to find "organisms, genes, or pathways that consistently explain the differences between two or more microbial communities" (Segata et al., 2011). Specifically, LEfSe uses rank-based Kruskal-Wallis (KW) sum-rank test to detect features with significant differential (relative) abundance with respect to the class of interest. Since it is rank-based, instead of proportional based, the differential species identified among the comparison groups is less biased (than percent abundance based).

Reference:

Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011 Jun 24;12(6):R60. doi: 10.1186/gb-2011-12-6-r60. PMID: 21702898; PMCID: PMC3218848.

case vs control

LEfSe Results for All Comparisons

Comparison No.	Comparison Name
Comparison 1.	case vs control
Comparison 2.	Male vs Female
Comparison 3.	no vs yes
Comparison 4.	no vs yes
Comparison 5.	no vs yes
Comparison 6.	no vs yes
Comparison 7.	no vs yes
Comparison 8.	no vs yes
Comparison 9.	Normal vs High
Comparison 10.	Normal vs High
Comparison 11.	Desirable vs Low
Comparison 12.	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal
Comparison 13.	Normal vs BorderlineHigh vs High
Comparison 14.	BorderlineHigh vs Normal vs High
Comparison 15.	High vs Normal
Comparison 16.	High vs Normal
Comparison 17.	Normal vs High

XI. Analysis - Heatmap Profile

Species vs Sample Abundance Heatmap for All Samples

Heatmaps for Individual Comparisons

A) Two-way clustering - clustered on both columns (Samples) and rows (organism)

Comparison No.	Comparison Name	Family Level		Genus Level		Species Level
Comparison 1	case vs control	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 2	Male vs Female	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 3	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 4	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 5	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 6	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 7	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 8	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 9	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 10	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 11	Desirable vs Low	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 12	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 13	Normal vs BorderlineHigh vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 14	BorderlineHigh vs Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 15	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 16	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 17	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG

B) One-way clustering - clustered on rows (organism) only

Comparison No.	Comparison Name	Family Level		Genus Level		Species Level
Comparison 1	case vs control	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 2	Male vs Female	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 3	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 4	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 5	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 6	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 7	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 8	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 9	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 10	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 11	Desirable vs Low	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 12	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 13	Normal vs BorderlineHigh vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 14	BorderlineHigh vs Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 15	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 16	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 17	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG

C) No clustering

Comparison No.	Comparison Name	Family Level		Genus Level		Species Level
Comparison 1	case vs control	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 2	Male vs Female	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 3	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 4	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 5	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 6	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 7	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 8	no vs yes	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 9	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 10	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 11	Desirable vs Low	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 12	High vs BorderlineHigh vs NearOptimal vs Very high vs Optimal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 13	Normal vs BorderlineHigh vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 14	BorderlineHigh vs Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 15	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 16	High vs Normal	PDF	SVG	PDF	SVG	PDF	SVG
Comparison 17	Normal vs High	PDF	SVG	PDF	SVG	PDF	SVG

XII. Analysis - Network Association

To analyze the co-occurrence or co-exclusion between microbial species among different samples, network correlation analysis tools are usually used for this purpose. However, microbiome count data are compositional. If count data are normalized to the total number of counts in the sample, the data become not independent and traditional statistical metrics (e.g., correlation) for the detection of specie-species relationships can lead to spurious results. In addition, sequencing-based studies typically measure hundreds of OTUs (species) on few samples; thus, inference of OTU-OTU association networks is severely under-powered. Here we use SPIEC-EASI (SParse InversE Covariance Estimation for Ecological Association Inference), a statistical method for the inference of microbial ecological networks from amplicon sequencing datasets that addresses both of these issues (Kurtz et al., 2015). SPIEC-EASI combines data transformations developed for compositional data analysis with a graphical model inference framework that assumes the underlying ecological association network is sparse. SPIEC-EASI provides two algorithms for network inferencing – 1) Meinshausen-Bühlmann's neighborhood selection (MB method) and inverse covariance selection (GLASSO method, i.e., graphical least absolute shrinkage and selection operator). This is fundamentally distinct from SparCC, which essentially estimate pairwise correlations. In addition to these two methods, we provide the results of a third method - SparCC (Sparse Correlations for Compositional Data)(Friedman & Alm 2012), which is also a method for inferring correlations from compositional data. SparCC estimates the linear Pearson correlations between the log-transformed components.

References:

Kurtz ZD, Müller CL, Miraldi ER, Littman DR, Blaser MJ, Bonneau RA. Sparse and compositionally robust inference of microbial ecological networks. PLoS Comput Biol. 2015 May 7;11(5):e1004226. doi: 10.1371/journal.pcbi.1004226. PMID: 25950956; PMCID: PMC4423992.

Friedman J, Alm EJ. Inferring correlation networks from genomic survey data. PLoS Comput Biol. 2012;8(9):e1002687. doi: 10.1371/journal.pcbi.1002687. Epub 2012 Sep 20. PMID: 23028285; PMCID: PMC3447976.

FOMC Service Report

16S rRNA Gene V1V3 Amplicon Sequencing

Version V1.41 fork

Version History

Project ID: 20250807_obesity

I. Project Summary

II. Workflow Checklist

III. NGS Sequencing

IV. Complete Report Download

V. Raw Sequence Data Download

VI. Analysis - DADA2 Read Processing

Sample Meta Information

ASV Read Counts by Samples

VII. Analysis - Read Taxonomy Assignment

Read Taxonomy Assignment - Methods

The species-level, open-reference 16S rRNA NGS reads taxonomy assignment pipeline

Read Taxonomy Assignment - Result Summary *

Read Taxonomy Assignment - ASV Species-Level Read Counts Table

Taxonomy Bar Plots for All Samples

Taxonomy Bar Plots for Individual Comparison Groups

VIII. Analysis - Alpha Diversity

Alpha Diversity Analysis by Rarefaction

Boxplot of Alpha-diversity Indices

Group Significance of Alpha-diversity Indices

IX. Analysis - Beta Diversity

NMDS and PCoA Plots

Interactive 3D PCoA Plots - Bray-Curtis Dissimilarity

Interactive 3D PCoA Plots - Euclidean Distance

Interactive 3D PCoA Plots - Correlation Coefficients

Group Significance of Beta-diversity Indices

X. Analysis - Differential Abundance

ANCOM Differential Abundance Analysis

ANCOM-BC Differential Abundance Analysis

LEfSe - Linear Discriminant Analysis Effect Size

XI. Analysis - Heatmap Profile

Species vs Sample Abundance Heatmap for All Samples

Heatmaps for Individual Comparisons

XII. Analysis - Network Association

SPIEC-EASI Network Inference by Neighborhood Selection (MB Method)

Association Network Inference by SparCC