FOMC 16S rRNA Sequence Analysis Pipeline Version History 2023-02-26 V1.43 Use ANCOMBC2 instead of ANCOMBC so for pairwise comparison for >= 3 groups 2022-11-17 V1.42 add abundance screenshot and information back 2022-04-12 v1.4 forked Forked away for FOMC unrelated projects 2022-03-24 V1.41 Fixed a bug in generating the OTU table file "final_otu_table_fullfax_all.txt". Now it is correctly generated based on final_otu_map_mc100.taxonomy and final_otu_map_mc100.txt 2022-02-04 V1.4 1. Added download for OTU tables with raw count, relative abundance and clr transformed abundance 2. Added heatmaps for 3 clustering options 1) no clustering 2) clustering on species only, 3) clustering on both species and samples 2021-12-17 V1.3 1. Change the comparison to be able to handle group comparison (e.g., not only pairwise comparision 2. Added QIIME 2 alpha-group-significance result 3. Added QIIME 2 beta-group-significance result 2021-11-10 V1.2 1. Added this version history to keep track of features added and bugs fixed 2. Added AncomBC differential abundance analsysis 3. Added results for groups in individual comparisons, such as alpha diversity box plots, beta diversity plots, top species/genus/family bar plots, and heatmaps. 4. Changed MC (minimal read count per species) to MPC (minimal percent read count per speices) to filter low abundant species by percent abundance of total reads. Currently set at MCP=0.1%, so species with fewer than total reads x 0.001 will be removed for downstream anaalyses. 2021-06-01 V1.1 1. Removed inclusion of the original Zymo absolute abundance plot 2021-03-15 V1.0 1. First version V1.0 released