FastQCFastQC Report
Tue 13 Jan 2026
zr26466_2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr26466_2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGTGGC67902.716No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTGTGGC57412.2963999999999998No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGGGGC41451.6580000000000001No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTGGGGC35961.4384No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC22140.8855999999999999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTGGG20860.8344No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGGG12390.49560000000000004No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGGGG8940.35760000000000003No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTGGGG8690.3476No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTGGGC7860.3144No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGGGC5120.20479999999999998No Hit
TCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGC4560.1824No Hit
CTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAG4500.18No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTATGGC4340.1736No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTAGG3940.1576No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTAGTGTGCC2950.11800000000000001No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGTGTC2880.1152No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTGGGGG2870.1148No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCAATTCATCTGGTATGGC2530.10120000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)