FastQCFastQC Report
Tue 13 Jan 2026
zr26466_4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr26466_4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGGGGGGC51962.0784000000000002No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGGGGGGG19040.7616No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGGGTGGC18790.7516No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGGGGGG14390.5756No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGGGGG13990.5596No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC10340.41359999999999997No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTAGGGGGGC7460.2984No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGTGTGGC6260.2504No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGTGGGGC5730.22920000000000001No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTAGGGGG4780.1912No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTGGGGTGGG4070.1628No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAATGGAGAATCTAGGGTGGC3380.1352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)