FastQCFastQC Report
Tue 13 Jan 2026
zr26466_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr26466_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTGTGGC51932.0772No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTATGGC26581.0632000000000001No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTATGCC17930.7172000000000001No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTGTGCC15900.636No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTATGCC13230.5292No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC12900.516No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTGTGGC11900.47600000000000003No Hit
TCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTATGC10490.41960000000000003No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTATGGC10070.40280000000000005No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTATG9640.3856No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTATG8830.3532No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTGTGCC7500.3No Hit
TCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTGTGC6370.25479999999999997No Hit
TCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTATGC5940.2376No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTGTG5560.2224No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTGGTGTGTC4860.1944No Hit
CTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAG3090.12359999999999999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTAGTGTG2850.11399999999999999No Hit
TCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGC2770.11080000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)