FastQCFastQC Report
Tue 30 Jun 2026
zr31987_15T_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr31987_15T_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41106832
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAGATC4157941.0114960938853181Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG586600.1427013397675598No Hit
AAGTAGCCTAAACCGGTATTGTTACGGCAGTGCCGGGGTTGTAGGACTGC554110.13479754411626757No Hit
TGATAGCTGGTTCTCCCCGAAATATATTGAGGTATAGCCTCAAGGATTTT506160.12313281646223674No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)