FastQCFastQC Report
Tue 30 Jun 2026
zr31987_2T_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr31987_2T_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36892555
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTGGTG763720.20701195674845507TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTGGGG580830.15743826904913472TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTAGTG437630.11862284951530194TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATAACCATCTAGTA436220.11824065858274116TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)