FastQCFastQC Report
Tue 30 Jun 2026
zr31987_3T_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr31987_3T_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36196450
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGTCTGCGGTGTAGATC2904140.8023273000529056Illumina Single End PCR Primer 1 (97% over 35bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA663160.18321133702338213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG647150.17878825133403967No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC401730.11098602210990305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)