FastQCFastQC Report
Tue 30 Jun 2026
zr31987_8T_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamezr31987_8T_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33353915
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATCGCACGTGTAGATC4881941.4636782518633868Illumina Single End PCR Primer 1 (96% over 33bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA715130.21440661463579314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG594210.17815299943050164No Hit
AAAGGTTTGGTCCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGC593860.17804806422274566No Hit
TAATCGCGGTCTATTATATATAGGCTGAGGAAAGTCCAAGCTTGCACAGG532070.15952250283062722No Hit
GAGGGGTCAAAAGATTCCCACAATTAGGGCGTCCAAACTGTTAGTCACTG511460.15334331816819705No Hit
AGTTTTCTGGGGTTGAGGACTGTGTAAGGTATAGAATGGAGATAGTTGAA334090.10016515302626393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)